西班牙北部野生和家养反刍动物宿主眼部的分子检测与鉴定
Molecular Detection and Identification of in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain.
作者信息
Dias-Alves Andrea, Cabezón Oscar, Borel Nicole, López-Olvera Jorge Ramón, Mentaberre Gregorio, Lavín Santiago, Fernández Aguilar Xavier
机构信息
Wildlife Conservation Medicine Research Group (WildCoM), Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain.
Institute of Veterinary Pathology, Department of Pathobiology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 268, CH-8057 Zurich, Switzerland.
出版信息
Pathogens. 2021 Mar 23;10(3):383. doi: 10.3390/pathogens10030383.
Infections by Chlamydiae are associated with ocular disease in humans and animals. In this study, the presence and diversity of spp. was assessed in diseased and healthy eyes of domestic sheep and wild ruminants that share mountain habitats in northern Spain. The presence of spp. was tested by real-time PCR in 1786 conjunctival swabs collected from both eyes of 893 animals from mountain habitats in northern Spain, and chlamydial species were identified in the positive samples by ArrayTube microarray methods. Chlamydial DNA was detected in 0.6% (CI95% 0.2-1.3) of the Pyrenean chamois () and 1.4% (CI95% <0.01-8.1) of the sheep () sampled, with the only chlamydial species identified. No association of with ocular disease or co-infection with was found. Further studies on the pathogenesis of infectious keratoconjunctivitis are needed to better understand the ecology of and its possible role as a ruminant pathogen at the wildlife-livestock interface.
衣原体感染与人类和动物的眼部疾病有关。在本研究中，评估了西班牙北部山区栖息地的家养绵羊和野生反刍动物患病和健康眼睛中衣原体属的存在情况和多样性。通过实时PCR检测了从西班牙北部山区栖息地的893只动物双眼采集的1786份结膜拭子中衣原体属的存在情况，并通过ArrayTube微阵列方法在阳性样本中鉴定衣原体种类。在采样的比利牛斯岩羚羊（）中，0.6%（95%置信区间0.2 - 1.3）检测到衣原体DNA，在绵羊（）中，1.4%（95%置信区间<0.01 - 8.1）检测到衣原体DNA，仅鉴定出为衣原体种类。未发现与眼部疾病有关联或与共感染。需要进一步研究感染性角膜结膜炎的发病机制，以更好地了解衣原体的生态学及其在野生动物 - 家畜界面作为反刍动物病原体的可能作用。
Zotero 插件