van der Mei Henny C, Rustema-Abbing Minie, Langworthy Don E, Collias Dimitris I, Mitchell Michael D, Bjorkquist Dave W, Busscher Henk J
Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
Biotechnol Bioeng. 2008 Jan 1;99(1):165-9. doi: 10.1002/bit.21538.
The attachment of waterborne pathogens onto surfaces can be increased by coating the surfaces with positive charge-enhancing polymers. In this paper, the increased efficacy of polydiallyldimethylammonium chloride (p-DADMAC) coatings on glass was evaluated in a parallel plate flow chamber with the use of waterborne pathogens (Raoultella terrigena, Escherichia coli, and Brevundimonas diminuta). p-DADMAC coatings strongly compensated the highly negative charges on the glass surface and even yielded a positively charged surface when applied from a 500 ppm solution. Whereas none of the strains adhered from water to glass due to electrostatic repulsion, R. terrigena and E. coli readily adhered in high numbers to p-DADMAC coated glass slides applied from 1, 100, or 500 ppm aqueous solutions. B. diminuta only adhered to a positively charged p-DADMAC coating applied from a 500 ppm solution. In addition, all p-DADMAC coatings indicated strong contact killing with the bacterial species used in this study by live/dead staining techniques. In summary, this paper demonstrates the potential of p-DADMAC coatings to strongly enhance bacterial adhesion. Moreover, once adhered, bacterial viability can be reduced by the positively charged ammonium groups in the coating.
通过用增强正电荷的聚合物涂覆表面，可以增加水传播病原体在表面上的附着。在本文中，使用水传播病原体（土生拉乌尔菌、大肠杆菌和短小鞘氨醇单胞菌）在平行板流动室中评估了聚二烯丙基二甲基氯化铵（p-DADMAC）涂层在玻璃上增强的效果。p-DADMAC涂层强烈补偿了玻璃表面的高负电荷，当从500 ppm溶液中涂覆时甚至产生了带正电荷的表面。由于静电排斥，没有菌株从水中附着到玻璃上，而土生拉乌尔菌和大肠杆菌很容易大量附着到从1、100或500 ppm水溶液中涂覆的p-DADMAC涂层载玻片上。短小鞘氨醇单胞菌仅附着到从500 ppm溶液中涂覆的带正电荷的p-DADMAC涂层上。此外，所有p-DADMAC涂层通过活/死染色技术表明对本研究中使用的细菌种类有很强的接触杀灭作用。总之，本文证明了p-DADMAC涂层有强大增强细菌附着的潜力。此外，一旦附着，涂层中带正电荷的铵基团可以降低细菌活力。
