Laboratório de Biologia Bucal, Centro de Ciências da Saúde, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
Departamento de Odontologia, Faculdade de Odontologia, Universidade de Pernambuco, Camaragibe, PE, Brasil.
Braz J Med Biol Res. 2020 Oct 30;53(12):e9468. doi: 10.1590/1414-431X20209468. eCollection 2020.
The aim of this study was to evaluate the antimicrobial activity and toxicity of glass ionomer cement (GIC) modified with 5-methyl-2-(1-methylethyl)phenol (thymol) against Streptococcus mutans in silico and in vitro. The antimicrobial activity of thymol on GIC modified with concentrations of 2% (GIC-2) and 4% (GIC-4) was evaluated in a model of planktonic cell biofilm using agar diffusion test, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), dynamic biofilm (continuous flow cell parallel), and bacterial kinetics. Conventional GIC (GIC-0) was used as a control. Thymol toxicity was evaluated in Artemia salina and in silico using Osiris® software. Differences between groups were estimated by analysis of variance, followed by Tukey post hoc test, with a 5% significance level. The results of the agar diffusion test between groups were not significantly different (P≥0.05). Thymol had potential bacteriostatic and bactericidal activity against Streptococcus mutans with respect to planktonic growth, with MIC of 100 µg/mL and MBC of 400 µg/mL. The groups GIC-0, GIC-2, and GIC-4 reduced the biofilm by approximately 10, 85, and 95%, respectively. Bacterial kinetics showed efficiency of the modified GICs for up to 96 h. GIC with thymol was effective against S. mutans, with significant inhibition of the biofilms. Analyses in silico and using Artemia salina resulted in no relevant toxicity, suggesting potential for use in humans. GIC-2 was effective against S. mutans biofilm, with decreased cell viability.
本研究旨在评估 5-甲基-2-(1-甲基乙基)苯酚（麝香草酚）改性玻璃离子水门汀（GIC）对变形链球菌的抗菌活性和毒性。采用琼脂扩散试验、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、动态生物膜（连续流动细胞平行）和细菌动力学，评估了浓度为 2%（GIC-2）和 4%（GIC-4）的麝香草酚改性 GIC 的抗菌活性。传统 GIC（GIC-0）用作对照。采用卤虫（Artemia salina）和 Osiris®软件进行麝香草酚毒性评估。通过方差分析估计组间差异，然后进行 Tukey 事后检验，显著性水平为 5%。组间琼脂扩散试验结果无显著差异（P≥0.05）。麝香草酚对浮游生长的变形链球菌具有潜在的抑菌和杀菌活性，MIC 为 100μg/mL，MBC 为 400μg/mL。GIC-0、GIC-2 和 GIC-4 组分别使生物膜减少约 10%、85%和 95%。细菌动力学表明改性 GIC 最长可达 96 小时有效。含麝香草酚的 GIC 对 S. mutans 有效，能显著抑制生物膜。利用 Artemia salina 和计算机分析均未发现相关毒性，提示其具有在人体中应用的潜力。GIC-2 对 S. mutans 生物膜有效，细胞活力降低。
