传统托盘反应器和GROWTEK生物反应器中α-淀粉酶生产的比较概况。
Comparative profiles of alpha-amylase production in conventional tray reactor and GROWTEK bioreactor.
作者信息
Bhanja Tapati, Rout S, Banerjee Rintu, Bhattacharyya B C
机构信息
Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India.
出版信息
Bioprocess Biosyst Eng. 2007 Sep;30(5):369-76. doi: 10.1007/s00449-007-0133-0. Epub 2007 Jun 18.
GROWTEK bioreactor was used as modified solid-state fermentor to circumvent many of the problems associated with the conventional tray reactors for solid-state fermentation (SSF). Aspergillus oryzae IFO-30103 produced very high levels of alpha-amylase by modified solid-state fermentation (mSSF) compared to SSF carried out in enamel coated metallic trays utilizing wheat bran as substrate. High alpha-amylase yield of 15,833 U g(-1) dry solid in mSSF were obtained when the fungus were cultivated at an initial pH of 6.0 at 32 degrees C for 54 h whereas alpha-amylase production in SSF reached its maxima (12,899 U g(-1) dry solid ) at 30 degrees C after 66 h of incubation. With the supplementation of 1% NaNO(3), the maximum activity obtained was 19,665 U g(-1) dry solid (24% higher than control) in mSSF, whereas, in SSF maximum activity was 15,480 U g(-1) dry solid in presence of 0.1% Triton X-100 (20% higher than the control).
GROWTEK生物反应器被用作改良固态发酵罐，以规避与传统固态发酵（SSF）托盘反应器相关的许多问题。与使用麦麸作为底物在搪瓷涂层金属托盘中进行的固态发酵相比，米曲霉IFO-30103通过改良固态发酵（mSSF）产生了非常高水平的α-淀粉酶。当真菌在初始pH为6.0、32℃下培养54小时时，mSSF中α-淀粉酶的高产率达到15,833 U g(-1)干固体，而在30℃培养66小时后，SSF中的α-淀粉酶产量达到最大值（12,899 U g(-1)干固体）。添加1%的NaNO(3)后，mSSF中获得的最大活性为19,665 U g(-1)干固体（比对照高24%），而在SSF中，在存在0.1% Triton X-100的情况下最大活性为15,480 U g(-1)干固体（比对照高20%）。
Zotero 插件