Plakas S M, DePaola A, Moxey M B
Food and Drug Administration, Fishery Research Branch, Dauphin Island, AL 36528.
J Assoc Off Anal Chem. 1991 Nov-Dec;74(6):910-2.
The Bacillus stearothermophilus disk assay for penicillin in milk (AOAC official method) was adapted for the determination of ampicillin in fish muscle. The method was evaluated in 2 species of cultured fish: channel catfish and striped bass. Recoveries of ampicillin ranged from 99 to 104% when muscle specimens from both species were spiked at concentrations of 0.025-1.00 micrograms/g. The lower limit of determination (LOD) was 0.025 micrograms/g. The assay was applied to monitor the elimination of ampicillin from the muscle of striped bass after intravascular administration (dosage of 10 mg/kg body weight). The mean concentrations in the muscle declined from 1.160 micrograms/g at 2 h to 0.063 micrograms/g at 18 h. The half-life of ampicillin in the muscle was 3.6 h. Ampicillin concentrations were below LOD at 24 h. No inhibitory activity was observed in the muscle of control fish.
用于测定牛奶中青霉素的嗜热脂肪芽孢杆菌纸片法（美国分析化学家协会官方方法）被改编用于测定鱼肉中的氨苄青霉素。该方法在两种养殖鱼类中进行了评估：斑点叉尾鮰和条纹鲈。当两种鱼类的肌肉样本添加浓度为0.025 - 1.00微克/克的氨苄青霉素时，回收率在99%至104%之间。测定下限（LOD）为0.025微克/克。该检测方法用于监测条纹鲈血管内给药（剂量为10毫克/千克体重）后肌肉中氨苄青霉素的消除情况。肌肉中的平均浓度从2小时时的1.160微克/克下降到18小时时的0.063微克/克。氨苄青霉素在肌肉中的半衰期为3.6小时。24小时时氨苄青霉素浓度低于测定下限。在对照鱼的肌肉中未观察到抑制活性。
