Lin Chih-Sheng, Kuo Chao-Lin, Wang Jui-Ping, Cheng Ju-Sang, Huang Zheng-Wen, Chen Chi-Fei
Department of Biological Science and Technology, National Chiao Tung University, 75 Po-Ai Street, Hsinchu 30050, Taiwan.
J Ethnopharmacol. 2007 Jul 25;112(3):557-67. doi: 10.1016/j.jep.2007.05.008. Epub 2007 May 10.
Perilla frutescens (L.) Britt. (Lamiaceae) has traditionally been used to treat diseases, including tumors, but the antitumorigenesis mechanism is unclear. We evaluated the effects of Perilla frutescens leaf extract (PLE) on proliferation and apoptosis inducing in human hepatoma HepG2 cells using a cell proliferation assay, flow cytometry, and cDNA microarrays. Gene expression and apoptosis were also assessed in HepG2 cells treated with a major constituent of PLE, rosmarinic acid (RosA). In the PLE-treated HepG2 cells, antiproliferative activity (105 microg/mL) were observed, flow cytometry revealed significant apoptosis, and microarray data indicated that the expression of a lot apoptosis-related genes were regulated in a time-dependent manner. Compared with PLE, RosA (10 microg/mL; a dose equivalent to 105 microg/mL of PLE) was less effective in increasing the expression of apoptosis-related genes and apoptosis inducing in HepG2 cells. Thus, additional PLE constituents may influence apoptosis in HepG2 cells. The results of our study suggest that the PLE should be further investigated as a promising to treat hepatocellular carcinoma.
紫苏（唇形科）传统上用于治疗包括肿瘤在内的疾病，但其抗肿瘤发生机制尚不清楚。我们使用细胞增殖测定、流式细胞术和cDNA微阵列评估了紫苏叶提取物（PLE）对人肝癌HepG2细胞增殖和凋亡诱导的影响。还用PLE的主要成分迷迭香酸（RosA）处理HepG2细胞，评估基因表达和凋亡情况。在用PLE处理的HepG2细胞中，观察到抗增殖活性（105微克/毫升），流式细胞术显示明显凋亡，微阵列数据表明许多凋亡相关基因的表达呈时间依赖性调节。与PLE相比，RosA（10微克/毫升；相当于105微克/毫升PLE的剂量）在增加HepG2细胞中凋亡相关基因的表达和诱导凋亡方面效果较差。因此，PLE的其他成分可能会影响HepG2细胞中的凋亡。我们的研究结果表明，PLE作为一种有前景的治疗肝细胞癌的药物应进一步研究。
