Yao Hong, Sun Yuanyuan, Lin Xinhua, Tang Yuhai, Liu Ailin, Li Guangwen, Li Wei, Zhang Shaobo
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, China.
Anal Sci. 2007 Jun;23(6):677-82. doi: 10.2116/analsci.23.677.
An electropolymerized film of eriochrome black T (EBT) has been prepared at a glassy carbon electrode (GCE) by cyclic voltammetry (CV). The poly(EBT) membrane at GCE exhibits an excellent electrocatalytic activity towards the oxidation of epinephrine (EP), ascorbic acid (AA) and uric acid (UA) in acidic solution and reduced the overpotential for the oxidation of EP. The poly(EBT)-coated electrode could separately detect EP, AA and UA in their mixture with the potential differences of 180 and 160 mV for EP-AA and UA-EP, respectively, which are large enough to allow for determination of EP in the presence of AA and UA. Using differential pulse voltammetry, the peak current of EP recorded in pH 3.5 solution was linearly dependent on EP's concentration in the range of 2.5 - 50 microM. Due to its good selectivity and stability, the polymer-coated GCE was successfully applied to the determination of EP in real samples.
通过循环伏安法（CV）在玻碳电极（GCE）上制备了铬黑T（EBT）的电聚合膜。GCE上的聚（EBT）膜对酸性溶液中肾上腺素（EP）、抗坏血酸（AA）和尿酸（UA）的氧化表现出优异的电催化活性，并降低了EP氧化的过电位。聚（EBT）涂层电极能够分别检测其混合物中的EP、AA和UA，EP与AA以及UA与EP之间的电位差分别为180和160 mV，这足以在存在AA和UA的情况下测定EP。使用差分脉冲伏安法，在pH 3.5溶液中记录的EP的峰值电流在2.5 - 50 microM范围内与EP的浓度呈线性关系。由于其良好的选择性和稳定性，聚合物涂层的GCE成功应用于实际样品中EP的测定。
