Miranda A G, Singh K V, Murray B E
Center for Infectious Diseases, University of Texas Medical School, Houston 77030.
J Clin Microbiol. 1991 Dec;29(12):2752-7. doi: 10.1128/jcm.29.12.2752-2757.1991.
Pulsed-field gel electrophoresis was used to compare 34 isolates of Enterococcus faecium from six different geographic locations. This procedure generated an average of 13 discernible fragment bands per isolate (range, 10 to 19 fragment bands) of 34 to 485 kb. The resulting restriction endonuclease digestion patterns were quite heterogeneous and were able to differentiate 27 of 34 isolates from each other, as defined by one or more mismatched fragment bands. Five patterns were shared by two or more isolates, and each set of isolates with matching patterns (shared pattern) originated in the same medical center, suggesting a common epidemiologic background, including highly penicillin resistant isolates in Richmond and Philadelphia. We conclude that pulsed-field gel electrophoresis of DNA digested with low-frequency-cleavage restriction enzymes offers a relatively simple method of comparing E. faecium for the purpose of epidemiologic study.
采用脉冲场凝胶电泳对来自六个不同地理位置的34株粪肠球菌进行比较。该方法对每株菌平均产生13条可分辨的片段带（范围为10至19条片段带），大小在34至485 kb之间。所得的限制性内切酶消化图谱差异很大，按照一条或多条不匹配的片段带定义，能够区分34株菌中的27株。有5种图谱为两个或更多菌株所共有，每组具有匹配图谱（共有图谱）的菌株都来自同一个医疗中心，提示有共同的流行病学背景，包括里士满和费城的高度耐青霉素菌株。我们得出结论，用低频切割限制性酶消化DNA的脉冲场凝胶电泳为进行粪肠球菌的流行病学研究比较提供了一种相对简单的方法。
