Anderson B E, Dawson J E, Jones D C, Wilson K H
Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1991 Dec;29(12):2838-42. doi: 10.1128/jcm.29.12.2838-2842.1991.
The bacterial 16S rRNA genes from blood samples of two patients with human ehrlichiosis and from an isolate recovered from one of the patients were amplified by using the polymerase chain reaction. The amplimers were then cloned and sequenced. The 16S rRNA gene sequence was also determined for Ehrlichia canis (two strains), E. equi, E. phagocytophila (two strains), and E. sennetsu (two strains). These sequences, along with a previously published 16S rRNA gene sequence of E. risticii, were compared. The 16S rRNA gene sequences were identical for all three sources of the human ehrlichiosis agent. The sequence comparisons indicate that the human ehrlichiosis agent is a new species most closely related to E. canis (98.2%) and more distantly related to other Ehrlichia spp. We propose that this species be named Ehrlichia chaffeensis sp. nov., with the Arkansas strain as the type strain.
利用聚合酶链反应扩增了两名人类埃立克体病患者血样中的细菌16S rRNA基因以及从其中一名患者身上分离出的菌株的该基因。然后对扩增产物进行克隆和测序。还测定了犬埃立克体（两个菌株）、马埃立克体、嗜吞噬细胞埃立克体（两个菌株）和腺热埃立克体（两个菌株）的16S rRNA基因序列。将这些序列与先前发表的里氏埃立克体16S rRNA基因序列进行了比较。人类埃立克体病病原体的所有三个来源的16S rRNA基因序列均相同。序列比较表明，人类埃立克体病病原体是一个新物种，与犬埃立克体关系最为密切（98.2%），与其他埃立克体属物种关系较远。我们建议将该物种命名为查菲埃立克体新种，以阿肯色菌株作为模式菌株。
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