Pusterla N, Huder J B, Feige K, Lutz H
Department of Veterinary Internal Medicine, University of Zurich, Switzerland.
J Clin Microbiol. 1998 Jul;36(7):2035-7. doi: 10.1128/JCM.36.7.2035-2037.1998.
This case report describes a 12-year-old Arabian mare with granulocytic ehrlichiosis. Clinical signs included fever, apathy, anorexia, icterus, limb edema, and reluctance to move. Examination of buffy coat smears revealed Ehrlichia organisms in neutrophils and eosinophils. A band of 1,428 bp was amplified from DNA of leukocytes via nested PCR and was identified as part of the Ehrlichia 16S rRNA gene. It differed from the gene sequences of Ehrlichia phagocytophila and E. equi at two and three positions, respectively. Interestingly, the nucleotide sequence of the 16S rRNA was 100% identical to that of the agent of human granulocytic ehrlichiosis.
本病例报告描述了一匹12岁的阿拉伯母马患粒细胞埃立克体病。临床症状包括发热、冷漠、厌食、黄疸、肢体水肿和不愿活动。对血沉棕黄层涂片的检查发现中性粒细胞和嗜酸性粒细胞中有埃立克体。通过巢式聚合酶链反应从白细胞DNA中扩增出一条1428bp的条带,并被鉴定为埃立克体16S rRNA基因的一部分。它与嗜吞噬细胞埃立克体和马埃立克体的基因序列分别在两个和三个位置不同。有趣的是,16S rRNA的核苷酸序列与人类粒细胞埃立克体病病原体的序列100%相同。