Anderson B E, Greene C E, Jones D C, Dawson J E
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Int J Syst Bacteriol. 1992 Apr;42(2):299-302. doi: 10.1099/00207713-42-2-299.
The 16S rRNA gene was amplified, cloned, and sequenced from the blood of two dogs that were experimentally infected with the etiologic agent of canine granulocytic ehrlichiosis. The 16S rRNA sequence was found to be unique when it was compared with the sequences of other members of the genus Ehrlichia. The most closely related species were Ehrlichia canis (98.0% related) and the human ehrlichiosis agent (Ehrlichia chaffeensis) (98.1% related); all other species in the genus were found to be phylogenetically much more distant. Our results, coupled with previous serologic data, provide conclusive evidence that the canine granulocytic ehrlichiosis agent is a new species of the genus Ehrlichia that is related to, but is distinct from, E. canis and all other members of the genus. We propose the name Ehrlichia ewingii sp. nov.; the Stillwater strain is the type strain.
从两只经实验感染犬粒细胞埃立克体病病原体的犬的血液中扩增、克隆并测序了16S rRNA基因。与埃立克体属其他成员的序列相比，发现该16S rRNA序列是独特的。与之关系最密切的物种是犬埃立克体（相关性为98.0%）和人埃立克体病病原体（恰菲埃立克体）（相关性为98.1%）；该属中的所有其他物种在系统发育上距离更远。我们的结果与先前的血清学数据相结合，提供了确凿证据，证明犬粒细胞埃立克体病病原体是埃立克体属的一个新物种，它与犬埃立克体及该属的所有其他成员相关，但又有所不同。我们提议将其命名为尤因埃立克体（Ehrlichia ewingii sp. nov.）；斯蒂尔沃特菌株为模式菌株。
