评估Vitek GPS-TA卡用于实验室检测肠球菌对高水平庆大霉素和链霉素的耐药性。
Evaluation of the Vitek GPS-TA card for laboratory detection of high-level gentamicin and streptomycin resistance in enterococci.
作者信息
Metchock B, McGowan J E
机构信息
Department of Pathology, Emory University School of Medicine, Atlanta, Georgia.
出版信息
J Clin Microbiol. 1991 Dec;29(12):2870-2. doi: 10.1128/jcm.29.12.2870-2872.1991.
Abstract
The Vitek GPS-TA card (Vitek Systems, Hazelwood, Mo.) was compared with single-concentration broth microdilution and disk diffusion methods using high-content disks for the detection of high-level resistance to gentamicin and streptomycin in 99 isolates of enterococci (81 Enterococcus faecalis isolates and 18 Enterococcus faecium isolates). The GPS-TA card accurately detected high-level resistance to gentamicin, but not streptomycin, in E. faecalis. When streptomycin is being considered for therapy, either disk diffusion or time-kill studies should be used to confirm susceptible results obtained by Vitek testing. Additional studies are needed to determine the best method for testing E. faecium isolates.
摘要
将Vitek GPS-TA卡（Vitek Systems公司，密苏里州黑兹尔伍德）与单浓度肉汤微量稀释法和使用高含量药敏纸片的纸片扩散法进行比较，以检测99株肠球菌（81株粪肠球菌和18株屎肠球菌）对庆大霉素和链霉素的高水平耐药性。GPS-TA卡在粪肠球菌中能准确检测出对庆大霉素的高水平耐药性，但不能检测对链霉素的高水平耐药性。当考虑使用链霉素进行治疗时，应采用纸片扩散法或时间杀菌试验来确认Vitek检测获得的敏感结果。需要进一步研究以确定检测屎肠球菌菌株的最佳方法。
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