Chiew Y F, Tosaka M, Yamane N
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
Diagn Microbiol Infect Dis. 1993 Feb;16(2):145-8. doi: 10.1016/0732-8893(93)90011-u.
A total of 250 strains of enterococci isolated in Kumamoto University Hospital, Japan, during the period from January to March 1992 were tested for high-level aminoglycoside resistance. Brain-heart infusion (BHI) agar plates supplemented with 1000 micrograms/ml of gentamicin or 2000 micrograms/ml of streptomycin detected 164 (66%) isolates resistant to either gentamicin or streptomycin alone, or both, and consisted of 107 (43%) resistant to gentamicin and 96 (38%) resistant to streptomycin. The Vitek Gram-Positive Susceptibility card (GPS-TA) revealed high correlations with those by agar screens, the results indicating a sensitivity of 100% and 99% to gentamicin and streptomycin, respectively, and 100% specificity to both. Also, the microdilution tests of the National Committee for Clinical Laboratory Standards (NCCLS) showed 100% and 92% sensitivity to gentamicin and streptomycin, respectively, and no false resistance (100% specificity) when compared with the results by agar screens.
1992年1月至3月期间，从日本熊本大学医院分离出的250株肠球菌进行了高水平氨基糖苷类耐药性检测。补充有1000微克/毫升庆大霉素或2000微克/毫升链霉素的脑心浸液（BHI）琼脂平板检测到164株（66%）对庆大霉素或链霉素单独耐药或两者均耐药的分离株，其中107株（43%）对庆大霉素耐药，96株（38%）对链霉素耐药。Vitek革兰氏阳性药敏卡（GPS-TA）与琼脂筛选结果高度相关，结果显示对庆大霉素和链霉素的敏感性分别为100%和99%，对两者的特异性均为100%。此外，美国国家临床实验室标准委员会（NCCLS）的微量稀释试验显示，与琼脂筛选结果相比，对庆大霉素和链霉素的敏感性分别为100%和92%，且无假耐药性（特异性为100%）。
