检测蛋黄与血清样本中鸡毒支原体抗体。
Detection of antibodies to Mycoplasma gallisepticum in egg yolk versus serum samples.
作者信息
Brown M B, Stoll M L, Scasserra A E, Butcher G D
机构信息
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611-0633.
出版信息
J Clin Microbiol. 1991 Dec;29(12):2901-3. doi: 10.1128/jcm.29.12.2901-2903.1991.
Serum (n = 1,636) and egg yolk (n = 802) samples collected from hens on four commercial egg farms in Florida were tested for the presence of specific antibodies to Mycoplasma gallisepticum in a commercially available enzyme-linked immunosorbent assay. No significant differences were noted between serum and egg yolk samples with respect to distribution of positive, suspect, and negative test results or for the mean sample/positive control ratio values of positive, suspect, and negative test results. A linear relationship between the distribution of positive and negative results and the age of the birds was observed for results obtained with both serum and egg yolk samples. On the basis of the results of this study, egg yolk samples can be used in lieu of serum samples to screen flocks for antibodies to Mycoplasma gallisepticum.
从佛罗里达州四个商业蛋鸡场的母鸡采集血清样本（n = 1636）和蛋黄样本（n = 802），采用市售酶联免疫吸附测定法检测鸡毒支原体特异性抗体的存在情况。血清和蛋黄样本在阳性、可疑和阴性检测结果的分布方面，以及阳性、可疑和阴性检测结果的平均样本/阳性对照比值方面，均未发现显著差异。血清和蛋黄样本的检测结果均显示，阳性和阴性结果的分布与鸡的年龄之间存在线性关系。基于本研究结果，蛋黄样本可替代血清样本用于筛选鸡群中的鸡毒支原体抗体。
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