Kati H, Sezen K, Demirbağ Z
Department of Biology, Faculty ofArts and Sciences, Giresun University, 28049 Giresun, Turkey.
Folia Microbiol (Praha). 2007;52(2):146-52. doi: 10.1007/BF02932153.
A bacterial isolate (Mm2) of Melolontha melolontha was identified and characterized. Based on various morphological, physiological, biochemical and molecular characteristics, it was identified as Bacillus thuringiensis subsp. tenebrionis. This isolate was compared to the reference strains by electron microscopy, SDS-PAGE analysis, plasmid pattern, cry gene content and insecticidal activity. Cells of the isolate harbored flat square inclusions containing a protein component of approximately equal to65 kDa. After trypsin digestion of solubilized crystals, SDS-PAGE resolved a unique proteinase-resistant peptide of approximately equal to 50 kDa. Plasmid pattern showed similar bands to those of the reference strain, PCR analysis showed that the isolate has cry3 gene. Toxicity tests (against 5 coleopteran species) showed 80 % insecticidal activity against the larvae of M. melolontha. The isolate Mm2 may be valuable as biological control agent for M. melolontha and other coleopteran insects.
对一种黄褐丽金龟的细菌分离株（Mm2）进行了鉴定和表征。基于各种形态学、生理学、生化和分子特征，它被鉴定为苏云金芽孢杆菌暗黑亚种。通过电子显微镜、SDS-PAGE分析、质粒图谱、cry基因含量和杀虫活性将该分离株与参考菌株进行了比较。该分离株的细胞含有扁平的方形内含物，其中含有一种约65 kDa的蛋白质成分。溶解的晶体经胰蛋白酶消化后，SDS-PAGE分离出一种约50 kDa的独特抗蛋白酶肽。质粒图谱显示与参考菌株的条带相似，PCR分析表明该分离株具有cry3基因。毒性试验（针对5种鞘翅目昆虫）显示对黄褐丽金龟幼虫有80%的杀虫活性。分离株Mm2作为黄褐丽金龟和其他鞘翅目昆虫的生物防治剂可能具有重要价值。
