Guerrero-Analco José, Medina-Campos Omar, Brindis Fernando, Bye Robert, Pedraza-Chaverri José, Navarrete Andrés, Mata Rachel
Facultad de Química, Universidad Nacional Autónoma de México, México DF, 04510, Mexico.
Phytochemistry. 2007 Aug;68(15):2087-95. doi: 10.1016/j.phytochem.2007.05.006. Epub 2007 Jun 18.
The extracts prepared from the stem barks of several Mexican copalchis species, including Hintonia latiflora, Exostema caribaeum and a commercial mixture of Hintonia standleyana and E. caribaeum (CM) showed significant hypoglycemic and antihyperglycemic effects. The extracts were tested in three different in vivo models using normal and streptozotocin (STZ)-induced diabetic rats. From the active extract of H. latiflora, 25-O-acetyl-3-O-beta-D-glucopyranosyl-23,24-dihydrocucurbitacin F (1), an analog of 23,24-dihydrocucurbitacin F, and several known compounds (2-8) were isolated; cucurbitacin 1 was also isolated from H. standleyana. Oral administration of H. latiflora extract [100mg/kg of body weight (bw)] and 5-O-beta-D-glucopyranosyl-7,3',4'-trihydroxy-4-phenylcoumarin (5) (30 mg/kg of bw) to STZ-induced diabetic rats, for a 30 day duration, restored blood glucose levels to normal values. The groups treated either with the active principle 5, or the extract of H. latiflora, showed less body weight loss than glibenclamide-treated and diabetic control groups (p<0.05). It was also demonstrated that the extract of H. latiflora regulated hepatic glycogen and plasma insulin levels (p<0.05). These data suggest that its antihyperglycemic effect is due in part to stimulation of insulin secretion and regulation of hepatic glycogen metabolism.
从几种墨西哥柯巴奇斯属植物的茎皮中提取的提取物，包括宽叶欣托木、加勒比外蕊木以及欣托木斯坦德利和加勒比外蕊木的商业混合物（CM），显示出显著的降血糖和抗高血糖作用。这些提取物在使用正常和链脲佐菌素（STZ）诱导的糖尿病大鼠的三种不同体内模型中进行了测试。从宽叶欣托木的活性提取物中，分离出了25 - O - 乙酰基 - 3 - O - β - D - 吡喃葡萄糖基 - 23,24 - 二氢葫芦素F（1），它是23,24 - 二氢葫芦素F的类似物，以及几种已知化合物（2 - 8）；葫芦素1也从斯坦德利欣托木中分离得到。对STZ诱导的糖尿病大鼠口服宽叶欣托木提取物[100mg/kg体重（bw）]和5 - O - β - D - 吡喃葡萄糖基 - 7,3',4'-三羟基 - 4 - 苯基香豆素（5）（30mg/kg bw），持续30天，可使血糖水平恢复到正常数值。用活性成分5或宽叶欣托木提取物处理的组，体重减轻比用格列本脲处理的组和糖尿病对照组少（p<0.05）。还证明了宽叶欣托木提取物调节肝糖原和血浆胰岛素水平（p<0.05）。这些数据表明，其抗高血糖作用部分归因于刺激胰岛素分泌和调节肝糖原代谢。
