原发性牙髓感染中协同菌属系统发育型的分子检测与鉴定
Molecular detection and identification of Synergistes phylotypes in primary endodontic infections.
作者信息
Siqueira J F, Rôças I N
机构信息
Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil.
出版信息
Oral Dis. 2007 Jul;13(4):398-401. doi: 10.1111/j.1601-0825.2006.01301.x.
AIM
Uncultivated phylotypes from the Synergistes group have recently emerged as suspected endodontic pathogens. This study aimed to investigate the presence and identity of these bacteria in primary endodontic infections using a 16S rRNA gene-based group-specific heminested polymerase chain reaction (PCR) protocol.
MATERIALS AND METHODS
Genomic DNA was isolated directly from clinical samples and used as templates for PCR. Amplicons from positive specimens were sequenced and phylogenetically analyzed to determine species identity.
RESULTS
Overall, about one-third of the samples harbored Synergistes bacteria. The following phylotypes were disclosed: oral clones W028, BA121/P4G_18 P1, W090, BH017 and E3_33.
CONCLUSIONS
These results demonstrated that as-yet uncultivated Synergistes phylotypes are present in the endodontic microbiota and a role in causation of apical periodontitis is suggested though remains unproven.
目的
来自协同菌属的未培养系统型最近已成为可疑的牙髓病病原体。本研究旨在使用基于16S rRNA基因的组特异性半巢式聚合酶链反应（PCR）方案，调查这些细菌在原发性牙髓感染中的存在情况和特性。
材料与方法
直接从临床样本中分离基因组DNA，并用作PCR模板。对阳性标本的扩增子进行测序和系统发育分析，以确定物种特性。
结果
总体而言，约三分之一的样本含有协同菌属细菌。发现了以下系统型：口腔克隆W028、BA121/P4G_18 P1、W090、BH017和E3_33。
结论
这些结果表明，牙髓微生物群中存在尚未培养的协同菌属系统型，提示其在根尖周炎病因中起作用，但仍未得到证实。
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