Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, UAE.
Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, UAE.
PLoS One. 2021 Aug 5;16(8):e0255485. doi: 10.1371/journal.pone.0255485. eCollection 2021.
To assess the prevalence of unculturable bacteria in periapical abscess, radicular cyst, and periapical granuloma.
PubMed, Scopus, Science Direct, and Ovid databases were systematically searched from January 1990 to May 2020. All the included studies were cross-sectional design. The risk of bias was assessed using Joanna Briggs Institute check-list. Heterogeneity was described using meta-regression and mixed-effects model for lesion, country, and sequence technique moderators. Funnel plot and unweighted Egger's regression test were used to estimate the publication bias. Microbiome data on diversity, abundance, and frequency of unculturable bacteria in the periapical lesions were reviewed, analysed, and the principal component analysis (PCA) was performed.
A total of 13 studies out of 14,780, were selected for the final analysis. These studies focused on the prevalence of unculturable bacteria in periapical abscesses and related lesions. Approximately 13% (95% CI: 7-23%) of the cumulative number of bacteria derived from periapical abscesses was unculturable. Country moderator significantly (P = 0.05) affects the diversity summary proportion. While the pooled frequency of unculturable bacteria was 8%; 95% CI: 5, 14%, the estimate of the pooled abundance of unculturable bacteria was 5%; 95% CI: 2, 12% with a significant (P = 0.05) country moderator that affects the abundance summary proportion. Of the 62 unculturable bacteria, 35 were subjected to PCA and Peptostreptococcus sp. oral clone CK035 was the most abundant species in periapical abscesses. Hybridization techniques were found to be the most reliable molecular methods in detecting the abundance and frequency of unculturable bacteria.
The significant prevalence of unculturable bacteria in the periapical abscess, suggests that they are likely to play, a yet unknown, critical role in the pathogenesis and progression of the disease. Further research remains to be done to confirm their specific contributions in the virulence and disease progression.
评估根尖脓肿、根侧囊肿和根尖肉芽肿中不可培养细菌的流行率。
系统检索了 1990 年 1 月至 2020 年 5 月的 PubMed、Scopus、Science Direct 和 Ovid 数据库。所有纳入的研究均为横断面设计。使用 Joanna Briggs 研究所清单评估偏倚风险。使用元回归和混合效应模型描述病变、国家和序列技术的异质性。使用漏斗图和未加权的 Egger 回归检验估计发表偏倚。对根尖病变中不可培养细菌的多样性、丰度和频率的微生物组数据进行了综述、分析，并进行了主成分分析（PCA）。
从 14780 篇文献中筛选出 13 项最终分析的研究。这些研究集中于根尖脓肿及相关病变中不可培养细菌的流行率。从根尖脓肿中获得的细菌中约有 13%（95%CI：7-23%）是不可培养的。国家因素显著（P=0.05）影响多样性总结比例。虽然不可培养细菌的 pooled 频率为 8%（95%CI：5,14%），不可培养细菌的 pooled 丰度估计值为 5%（95%CI：2,12%），但国家因素显著（P=0.05）影响丰度总结比例。在 62 种不可培养细菌中，有 35 种进行了 PCA，口腔定植菌 Peptostreptococcus sp. oral clone CK035 是根尖脓肿中最丰富的物种。杂交技术被发现是检测不可培养细菌丰度和频率的最可靠的分子方法。
根尖脓肿中不可培养细菌的显著流行率表明，它们可能在疾病的发病机制和进展中发挥尚未可知的关键作用。需要进一步研究来确认它们在毒力和疾病进展中的具体贡献。
