Li Haiyan, Zhai Wanying, Chang Jiang
Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, P.R. China.
J Mater Sci Mater Med. 2008 Jan;19(1):67-73. doi: 10.1007/s10856-007-3170-y. Epub 2007 Jun 19.
Biodegradable and biocompatible materials are the basis for tissue engineering. As an initial step for developing bone tissue engineering scaffolds, the in vitro biocompatibility of degradable and bioactive composites consisting of polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and wollastonite (W) was studied by culturing osteoblasts on the PHBV/W substrates, and the cell adhesion, morphology, proliferation, and alkaline phosphatase (ALP) activity were evaluated. The results showed that the incorporation of wollastonite benefited osteoblasts adhesion and the osteoblasts cultured on the PHBV/W composite substrates spread better as compared to those on the pure PHBV after culturing for 3 h. In the prolonged incubation time, the osteoblasts cultured on the PHBV/W composite substrates revealed a higher proliferation and differentiation rate than those on the pure PHBV substrates. In addition, an increase of proliferation and differentiation rate was observed when the wollastonite content in the PHBV/W composites increased from 10 to 20 wt%. All of the results showed that the addition of wollastonite into PHBV could stimulate osteoblasts to proliferate and differentiate and the PHBV/W composites with wollastonite up to 20 wt% were more compatible than the pure PHBV materials for bone repair and bone tissue engineering.
可生物降解和生物相容的材料是组织工程的基础。作为开发骨组织工程支架的第一步，通过在聚羟基丁酸酯-共-羟基戊酸酯（PHBV）/硅灰石（W）基底上培养成骨细胞，研究了由PHBV和硅灰石组成的可降解生物活性复合材料的体外生物相容性，并评估了细胞黏附、形态、增殖和碱性磷酸酶（ALP）活性。结果表明，硅灰石的加入有利于成骨细胞黏附，培养3小时后，在PHBV/W复合基底上培养的成骨细胞比在纯PHBV基底上培养的成骨细胞铺展得更好。在延长的孵育时间内，在PHBV/W复合基底上培养的成骨细胞比在纯PHBV基底上培养的成骨细胞显示出更高的增殖和分化率。此外，当PHBV/W复合材料中硅灰石含量从10 wt%增加到20 wt%时，观察到增殖和分化率增加。所有结果表明，向PHBV中添加硅灰石可刺激成骨细胞增殖和分化，含硅灰石高达20 wt%的PHBV/W复合材料比纯PHBV材料在骨修复和骨组织工程方面更具相容性。
