聚（3-羟基丁酸酯-co-3-羟基己酸酯）对骨髓基质细胞亲和力的研究
Studies on bone marrow stromal cells affinity of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate).
作者信息
Yang Ming, Zhu Shanshan, Chen Yue, Chang Zhijie, Chen Guoqiang, Gong Yandao, Zhao Nanming, Zhang Xiufang
机构信息
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
出版信息
Biomaterials. 2004 Mar-Apr;25(7-8):1365-73. doi: 10.1016/j.biomaterials.2003.08.018.
The objective of this study is to investigate the biocompatibility of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) with bone marrow stromal cells in vitro. The adsorption of fibronectin on the material was studied by enzyme-linked immunosorbent assay. After bone marrow stromal cells were seeded and cultured on PHBHHx, their proliferation was investigated by MTT. Differentiation of the cells was assessed by measuring alkaline phosphatase activity and by histochemical assay. The wettability and thermal property of PHBHHx films were also studied by contact angle goniometer, thermogravimetry and differential scanning calorimetry, respectively. The results show that bone marrow stromal cells can attach, proliferate and differentiate into osteoblasts on PHBHHx films. These results suggest that PHBHHx has good affinity with bone marrow stromal cells and may have potential applications in bone tissue engineering.
本研究的目的是在体外研究聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）与骨髓基质细胞的生物相容性。通过酶联免疫吸附测定法研究纤连蛋白在该材料上的吸附。将骨髓基质细胞接种在PHBHHx上并进行培养后，通过MTT法研究其增殖情况。通过测量碱性磷酸酶活性和组织化学测定法评估细胞的分化情况。还分别通过接触角测量仪、热重分析法和差示扫描量热法研究了PHBHHx薄膜的润湿性和热性能。结果表明，骨髓基质细胞能够在PHBHHx薄膜上附着、增殖并分化为成骨细胞。这些结果表明，PHBHHx与骨髓基质细胞具有良好的亲和力，并且可能在骨组织工程中有潜在应用。
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