Lee Bo-Bae, Cha Mi-Ran, Kim Soo-Yeon, Park Eunju, Park Hae-Ryong, Lee Seung-Cheol
Department of Food Science and Biotechnology, Kyungnam University, Masan, Republic of Korea.
Plant Foods Hum Nutr. 2007 Jun;62(2):79-84. doi: 10.1007/s11130-007-0045-9. Epub 2007 Jun 19.
Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 microg/ml) showed the highest total phenol content (104.30 microM), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE on DNA damage induced by H(2)O(2) in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by 200 microM of H(2)O(2), methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 microg/ml showed 38.8% inhibition against growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive materials.
制备了樱桃（野生山樱花）花的有机溶剂（甲醇、乙醇和丙酮）提取物和水提取物，并对提取物的抗氧化活性进行了评估。甲醇提取物CBE（100微克/毫升）的总酚含量最高（104.30微摩尔）、自由基清除活性最强（34.2%）、还原力最高（0.391）。通过彗星试验评估了CBE对H₂O₂诱导的人白细胞DNA损伤的影响。所有CBE均是200微摩尔H₂O₂诱导的DNA损伤的有效剂量依赖性抑制剂，甲醇提取物CBE的抑制活性最强。500微克/毫升的甲醇提取物CBE对人结肠癌细胞系HT - 29的生长有38.8%的抑制作用。这些结果表明，樱花可以提供有价值的生物活性物质。
