Yang Li-Quan, Dai Xiao-Jun, Luo Yuan-Ming, Ma Chun-Xiao, Hou Jian-Hu, Wu Zhi-Qiang, Wang Cui-Yan, Li Ming-Gang
The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071, China.
Sheng Wu Gong Cheng Xue Bao. 2007 May;23(3):477-82, 524.
According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
根据报道的米根霉糖化酶基因序列，从野生型少根根霉的总DNA中克隆出含有4个内含子的糖化酶基因。设计特异性引物通过重叠PCR删除内含子，获得少根根霉糖化酶的新cDNA序列。该基因在基因库中的登录号为DQ903853。该基因在毕赤酵母中成功表达，产生了一种具有高活性糖化酶的新蛋白。
