来自多重抑制研究的关于柠檬酸合酶的等排体和别构核苷酸抑制作用的证据。
Evidence of isosteric and allosteric nucleotide inhibition of citrate synthease from multiple-inhibition studies.
作者信息
Harford S, Weitzman P D
出版信息
Biochem J. 1975 Nov;151(2):455-8. doi: 10.1042/bj1510455.
Abstract
Citrate synthases from diverse organisms are inhibited by ATP and NADH. Evidence is presented, from multiple-inhibition studies on various citrate synthases, that ATP acts in all cases as an isosteric inhibitor at the acetyl-CoA site. On the other hand, NADH also acts isosterically with eukaryotic and Gram-positive bacterial citrate synthases, but behaves as an allosteric inhibitor specifically in the case of the Gram-negative bacterial enzyme. After desensitization to this allosteric inhibition, only the isosteric nucleotide inhibition, as found in other citrate syntheases, is observed.
摘要
来自不同生物体的柠檬酸合酶受到ATP和NADH的抑制。通过对各种柠檬酸合酶的多重抑制研究表明，在所有情况下，ATP在乙酰辅酶A位点作为等构抑制剂起作用。另一方面，NADH对真核生物和革兰氏阳性细菌的柠檬酸合酶也起等构作用，但仅在革兰氏阴性细菌酶的情况下表现为别构抑制剂。在对这种别构抑制脱敏后，只观察到如在其他柠檬酸合酶中发现的等构核苷酸抑制作用。
相似文献
1
Evidence of isosteric and allosteric nucleotide inhibition of citrate synthease from multiple-inhibition studies.来自多重抑制研究的关于柠檬酸合酶的等排体和别构核苷酸抑制作用的证据。
Biochem J. 1975 Nov;151(2):455-8. doi: 10.1042/bj1510455.
2
Kinetics of the inhibition of citrate synthase from pig heart by substrate analogues.底物类似物对猪心柠檬酸合酶的抑制动力学。
Eur J Biochem. 1974 Jul 1;46(1):5-11. doi: 10.1111/j.1432-1033.1974.tb03591.x.
3
Regulation of citrate synthase activity in methylotrophs by reduced nicotinamide-adenine dinucleotide, adenine nucleotides and 2-oxoglutarate.还原型烟酰胺腺嘌呤二核苷酸、腺嘌呤核苷酸和2-氧代戊二酸对甲基营养菌中柠檬酸合酶活性的调节
Biochem J. 1975 Jul;150(1):141-4. doi: 10.1042/bj1500141.
4
Citrate synthase from Crithidia fasciculata: inhibition by adenine nucleotides and suramin.来自克氏锥虫的柠檬酸合酶：被腺嘌呤核苷酸和苏拉明抑制
Comp Biochem Physiol B. 1982;72(1):165-8. doi: 10.1016/0305-0491(82)90029-3.
5
The interactions of adenylates with allosteric citrate synthase.
Can J Biochem. 1979 May;57(5):385-95. doi: 10.1139/o79-049.
6
A comparison of the citrate synthases of Escherichia coli and Acinetobacter anitratum.大肠杆菌和反硝化不动杆菌柠檬酸合酶的比较。
Can J Biochem. 1980 Sep;58(9):696-706. doi: 10.1139/o80-098.
7
A new spectrophotometric assay for citrate synthase and its use to assess the inhibitory effects of palmitoyl thioesters.一种用于柠檬酸合酶的新型分光光度测定法及其用于评估棕榈酰硫酯抑制作用的用途。
Biochem J. 1988 May 1;251(3):803-7. doi: 10.1042/bj2510803.
8
Models of proteolysis of oligomeric enzymes and their applications to the trypsinolysis of citrate synthases.寡聚酶蛋白水解模型及其在柠檬酸合酶胰蛋白酶水解中的应用。
Biochem J. 1988 Sep 1;254(2):437-42. doi: 10.1042/bj2540437.
9
Inhibition of pig-heart citrate synthase by carboxylic acids structurally related to oxaloacetate.与草酰乙酸结构相关的羧酸对猪心柠檬酸合酶的抑制作用。
Eur J Biochem. 1979 Feb 1;93(3):505-13. doi: 10.1111/j.1432-1033.1979.tb12849.x.
10
Citrate synthase from a Gram-positive bacterium. Purification and characterization of the Bacillus megaterium enzyme.来自革兰氏阳性细菌的柠檬酸合酶。巨大芽孢杆菌酶的纯化与特性研究
Biochem J. 1983 Jul 1;213(1):53-9. doi: 10.1042/bj2130053.
引用本文的文献
1
The Multienzyme Complex Nature of Dehydroepiandrosterone Sulfate Biosynthesis.硫酸脱氢表雄酮生物合成的多酶复合物性质。
Int J Mol Sci. 2024 Feb 8;25(4):2072. doi: 10.3390/ijms25042072.
2
Structural and biochemical characterization of mitochondrial citrate synthase 4 from Arabidopsis thaliana.拟南芥线粒体柠檬酸合酶4的结构与生化特性
Acta Crystallogr F Struct Biol Commun. 2020 Mar 1;76(Pt 3):109-115. doi: 10.1107/S2053230X20001521.
3
Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.金属球菌属柠檬酸合酶抑制特性的结构研究
PLoS One. 2019 Feb 22;14(2):e0212807. doi: 10.1371/journal.pone.0212807. eCollection 2019.
4
Citrate synthase from a Gram-positive bacterium. Purification and characterization of the Bacillus megaterium enzyme.来自革兰氏阳性细菌的柠檬酸合酶。巨大芽孢杆菌酶的纯化与特性研究
Biochem J. 1983 Jul 1;213(1):53-9. doi: 10.1042/bj2130053.
5
Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa.铜绿假单胞菌对NADH敏感的柠檬酸合酶基因的克隆、测序及表达
J Bacteriol. 1989 Oct;171(10):5542-50. doi: 10.1128/jb.171.10.5542-5550.1989.
6
Cloning and nucleotide sequence of the gene coding for citrate synthase from a thermotolerant Bacillus sp.一株耐热芽孢杆菌柠檬酸合酶编码基因的克隆及核苷酸序列分析
Appl Environ Microbiol. 1992 Jan;58(1):335-45. doi: 10.1128/aem.58.1.335-345.1992.
7
Regulation of citrate synthase from blue-green bacteria by succinyl coenzyme A.琥珀酰辅酶A对蓝细菌柠檬酸合酶的调控
Arch Microbiol. 1977 Jul 26;114(1):55-60. doi: 10.1007/BF00429630.
本文引用的文献
1
STUDIES ON LIVER ALCOHOL HYDROGENASE COMPLEXES. 3. MULTIPLE INHIBITION KINETICS IN THE PRESENCE OF TWO COMPETITIVE INHIBITORS.肝脏乙醇脱氢酶复合物的研究。3. 两种竞争性抑制剂存在下的多重抑制动力学
Arch Biochem Biophys. 1964 Jul 20;106:243-51. doi: 10.1016/0003-9861(64)90184-5.
2
Kinetics of regulatory enzymes: effect of adenosine triphosphate on yeast citrate synthase.调节酶的动力学：三磷酸腺苷对酵母柠檬酸合酶的影响
Biochem Biophys Res Commun. 1965 Sep 8;20(5):661-5. doi: 10.1016/0006-291x(65)90452-3.
3
Conditions for the self-catalysed inactivation of carnitine acetyltransferase. A novel form of enzyme inhibition.肉碱乙酰转移酶自催化失活的条件。一种新型酶抑制形式。
Biochem J. 1969 Jan;111(2):225-35. doi: 10.1042/bj1110225.
4
Controls of citrate synthase activity.
Life Sci. 1974 Nov 15;15(10):1695-710. doi: 10.1016/0024-3205(74)90172-6.
5
The citrate enzymes: their structures, mechanisms, and biological functions.柠檬酸酶：它们的结构、作用机制及生物学功能
Curr Top Cell Regul. 1972;5:229-83. doi: 10.1016/b978-0-12-152805-8.50013-7.
6
Studies on purified citrate-enzymes: metabolic interpretations.纯化柠檬酸酶的研究：代谢解读
Biochem Soc Symp. 1968;27:11-21.
7
Citrate synthases: allosteric regulation and molecular size.柠檬酸合酶：变构调节与分子大小
Biochim Biophys Acta. 1969 Jan 7;171(1):198-200. doi: 10.1016/0005-2744(69)90122-3.
8
Regulation of citrate synthase and microbial taxonomy.柠檬酸合酶的调控与微生物分类学
Nature. 1968 Jul 20;219(5151):270-2. doi: 10.1038/219270a0.
Zotero 插件