Wang J, Wang J, Zhang H, Ma H, Li Y, Li J
Nanjing University School of Medicine, Department of General Surgery, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, China.
Transplant Proc. 2007 Jun;39(5):1637-9. doi: 10.1016/j.transproceed.2006.12.038.
The aim of this study was to assess the influence of long-term administration of cyclosporine (CsA) on rat intestinal intraepithelial lymphocytes (IEL). Ten male Lewis rats were randomly assigned to either a treatment or a control group. For 12 weeks the treatment group received CsA (5 mg/kg per day) while the control group received the same volume of vehicle. Peripheral blood lymphocytes were counted and IEL were isolated for flow cytometric analysis. Peripheral blood lymphocytes numbers were significantly decreased in the CsA compared with the control group (4.80 +/- 1.02 x 10(6)/mL and 3.34 +/- 0.70 x 10(6)/mL, respectively, P = .029). IEL numbers were increased significantly in CsA compared with the control group. (23.2 +/- 8.0 x 10(6) and 7.8 +/- 1.8 x 10(6), respectively, P = .012). There were no significant differences between the two groups considering IEL phenotypes. In conclusion, CsA did not influence the subpopulation but significantly increased the number of IEL.
本研究的目的是评估长期给予环孢素（CsA）对大鼠肠道上皮内淋巴细胞（IEL）的影响。将10只雄性Lewis大鼠随机分为治疗组和对照组。治疗组连续12周给予CsA（每天5 mg/kg），而对照组给予相同体积的赋形剂。计数外周血淋巴细胞，并分离IEL进行流式细胞术分析。与对照组相比，CsA组外周血淋巴细胞数量显著减少（分别为4.80±1.02×10⁶/mL和3.34±0.70×10⁶/mL，P = 0.029）。与对照组相比，CsA组IEL数量显著增加（分别为23.2±8.0×10⁶和7.8±1.8×10⁶，P = 0.012）。考虑IEL表型，两组之间无显著差异。总之，CsA不影响亚群，但显著增加了IEL的数量。
