利用聚合酶链反应（PCR）和限制性酶切技术检测沙特阿拉伯吉赞地区氯喹和乙胺嘧啶-磺胺多辛的耐药性标志物
Detection of drug resistance markers for chloroquine and pyrimethamine-sulfadoxine in Jazan area, Saudi Arabia using PCR and restriction digestion.
作者信息
Al Harthi Saeed A
机构信息
Department of Medical Parasitology, Faculty of Medicine, Umm Al-Qura University, P.O. Box 13955, Makkah, Saudi Arabia.
出版信息
J Egypt Soc Parasitol. 2007 Apr;37(1):17-30.
Nested PCR and restriction analysis were used to detect mutations at codon 76 of Plasmodium falciparum chloroquine resistance transporter gene (pfcrt) and codon 59 of dihydrofolate reductase gene (dhfr) that indicate chloroquine (CQ) and pyrimethamine-sulfadoxine (PYR-SDX) resistance respectively. P. falciparum isolates from malaria-endemic area of Jazan showed CQ resistance rate (89.5%), the highest percentage of chloroquine resistance ever recorded in Saudi Arabia. One the other hand, 10.5% of isolates showed a PYR-SDX resistant allele as a first reported in the kingdom. The use of molecular markers as additional tools to map areas of chloroquine resistance was expected to contribute in the development of new strategies for therapeutic intervention towards malaria in Saudi Arabia.
采用巢式聚合酶链反应（Nested PCR）和限制性分析来检测恶性疟原虫氯喹抗性转运蛋白基因（pfcrt）第76位密码子和二氢叶酸还原酶基因（dhfr）第59位密码子的突变，这些突变分别指示对氯喹（CQ）和乙胺嘧啶-磺胺多辛（PYR-SDX）的抗性。来自吉赞疟疾流行区的恶性疟原虫分离株显示出CQ抗性率（89.5%），这是沙特阿拉伯有记录以来氯喹抗性的最高百分比。另一方面，10.5%的分离株显示出PYR-SDX抗性等位基因，这在沙特王国尚属首次报道。使用分子标记作为绘制氯喹抗性区域的额外工具，有望为沙特阿拉伯疟疾治疗干预新策略的制定做出贡献。
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