Lipari Elvira Farina, Lipari Alessio, Dieli Francesco, Valentino Biagio
Dipartimento di Medicina Sperimentale, Sezione Anatomia umana, Università di Palermo.
Ital J Anat Embryol. 2007 Jan-Mar;112(1):19-25.
In previous research, we studied both the oxytocin and vasopressin ontogeny in the hypothalamic supraoptic and paraventricular nuclei, and the ANP-ontogeny in the hypothalamic supraoptic nucleus. In this paper we evaluate the ANP-ontogeny in the rat hypothalamic suprachiasmatic nucleus; infact the suprachiasmatic nucleus it is known to synthesize vasopressin, a peptidic hormone involved in the homeostasis of the body fluids by an antagonistic role to ANP. Immunohistochemical techniques show that ANP is present in the hypothalamic suprachiasmatic nucleus of the rat at 18 degrees day of i.u. life and at 0 degrees to 3 degrees day of postnatal life. PCR analysis confirms the ANP-mRNA expression. Thus, it is possible to adfirm that the suprachiasmatic nucleus is a synthesis site of ANP, and ANP appears in both the supraoptic and suprachiasmatic nuclei at the same developmental stage. Moreover, ANP and vasopressin appear at the same developmental stage since both the peptides are involved in the homeostasis of body fluids.
在先前的研究中，我们研究了下丘脑视上核和室旁核中催产素和加压素的个体发生，以及下丘脑视上核中的心钠素个体发生。在本文中，我们评估大鼠下丘脑视交叉上核的心钠素个体发生；事实上，已知视交叉上核可合成加压素，这是一种肽类激素，通过与心钠素的拮抗作用参与体液平衡。免疫组织化学技术显示，在宫内生活第18天以及出生后0至3天的大鼠下丘脑视交叉上核中存在心钠素。聚合酶链反应分析证实了心钠素mRNA的表达。因此，可以确定视交叉上核是心钠素的合成部位，并且心钠素在相同的发育阶段出现在视上核和视交叉上核中。此外，心钠素和加压素在相同的发育阶段出现，因为这两种肽都参与体液平衡。
