Ou Junjie, Dong Jing, Wu Minghuo, Kong Liang, Zou Hanfa
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Se Pu. 2007 Mar;25(2):129-34.
The molecularly imprinted polymer (MIP) monolithic column was firstly in situ polymerized in a traditional stainless-steel chromatographic column by using methylacrylic acid and S-Tröger's base as the functional monomer and imprinted template molecules, respectively. The effects of the content of acetic acid and water in mobile phase on the separation were systematically investigated. The fast enantioseparation of the racemate on monolithic column was successfully realized by stepwise gradient elution. The MIP monolithic capillary column was also synthesized in a fused-silica capillary by in situ polymerization with 2-( dimethylamino) ethyl methacrylate (DAMA) as functional monomer. The enantioseparation of 1,1'-bi-2-naphthol (BNL) on the obtained MIP capillary column was achieved in capillary electrochromatography (CEC). The results indicated that other acidic template can be imprinted by using basic DAMA as functional monomer, which enlarge the application fields of imprinting molecules.
分子印迹聚合物（MIP）整体柱首先在传统不锈钢色谱柱中原位聚合，分别使用甲基丙烯酸和S-特罗格碱作为功能单体和印迹模板分子。系统研究了流动相中乙酸和水的含量对分离的影响。通过梯度洗脱成功实现了外消旋体在整体柱上的快速对映体分离。还以甲基丙烯酸2-（二甲氨基）乙酯（DAMA）为功能单体，通过原位聚合在熔融石英毛细管中合成了MIP整体毛细管柱。在毛细管电色谱（CEC）中实现了1,1'-联-2-萘酚（BNL）在所得MIP毛细管柱上的对映体分离。结果表明，使用碱性DAMA作为功能单体可以印迹其他酸性模板，这扩大了印迹分子的应用领域。
