Li Min, Lin Xucong, Xie Zenghong
Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
J Chromatogr A. 2009 Jul 3;1216(27):5320-6. doi: 10.1016/j.chroma.2009.05.012. Epub 2009 May 15.
Molecularly imprinted monolithic columns were prepared for chiral separation of tyrosine and its amino acid derivatives by in situ therm-initiated copolymerization of methacrylic acid, 4-vinylpyridine and ethylene glycol dimethacrylate. The enantiomers were rapidly separated on monolithic columns in less than 10 min by pressurized capillary electrochromatography (pCEC). The influences of several parameters such as the content of cross-linking monomer on the composition of the pre-polymerization mixture were systematically investigated. The influence of the pCEC conditions including the composition of the mobile phase was also optimized to obtain the good enantioseparation. It was found that in addition to molecularly imprinted recognition, chromatographic retention and electrophoretic migration play important roles in the retention and chiral recognition of molecularly imprinted polymer (MIP) columns. The cross-selectivity for similar amino acids and its derivatives were systematical investigated for understanding the recognition mechanism on the MIP monolithic columns. The results indicated that molecularly imprinted polymer recognizes the template molecule by its molecular shape defined binding cavity.
通过甲基丙烯酸、4-乙烯基吡啶和乙二醇二甲基丙烯酸酯的原位热引发共聚反应，制备了用于酪氨酸及其氨基酸衍生物手性分离的分子印迹整体柱。通过加压毛细管电色谱（pCEC），对映体在整体柱上不到10分钟即可快速分离。系统研究了几个参数的影响，如交联单体含量对预聚合混合物组成的影响。还优化了包括流动相组成在内的pCEC条件的影响，以实现良好的对映体分离。研究发现，除了分子印迹识别外，色谱保留和电泳迁移在分子印迹聚合物（MIP）柱的保留和手性识别中也起着重要作用。系统研究了对相似氨基酸及其衍生物的交叉选择性，以了解MIP整体柱上的识别机制。结果表明，分子印迹聚合物通过其分子形状定义的结合腔识别模板分子。
