Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
J Chromatogr A. 2011 Sep 16;1218(37):6498-504. doi: 10.1016/j.chroma.2011.07.012. Epub 2011 Jul 18.
One monomer molecularly imprinted polymer coatings were first synthesized in fused silica capillary columns with 2-methacrylamidopropyl methacrylate (MAM) as single functional monomer in addition to a cross-linking monomer. Since MAM may generate no or little EOF, a strategy of precursor of polymerization, which does not interfere with the formation of defined imprints, was used to introduce an ionizable functional monomer to generate a stable electroosmotic flow for electrochromatography (CEC) by post-polymerization hydrolization. The resulting MAM-based open-tubular imprinted capillary was able to separate enantiomers by means of CEC. The resolution of enantiomers separation achieved on S-amlodipine-imprinted capillary was up to 16.1. The strong recognition ability (selectivity factor was 3.23) and high column performance (theory plates was 26,053 plates m(-1)) of template were obtained. The MIP coatings were also prepared using either S-naproxen or S-ketoprofen as template molecule. The resolutions of enantiomers separation were 2.20 and 4.56, respectively. The results illustrate that the synthesis of MIP using one monomer is not only an experimental-simplified process, but also an approach to producing chiral stationary phase with high efficiency and selectivity.
首先，以 2-丙烯酰胺基丙基甲基丙烯酰胺（MAM）作为单官能单体，在熔融石英毛细管柱中合成了一种单体分子印迹聚合物涂层，此外还有一种交联单体。由于 MAM 可能不会或很少产生电渗流（EOF），因此采用聚合前体策略，该策略不会干扰定义印迹的形成，通过聚合后水解将可离子化的官能单体引入到毛细管中，以生成用于电色谱（CEC）的稳定电渗流。所得的基于 MAM 的开管印迹毛细管能够通过 CEC 分离对映体。在 S-氨氯地平印迹毛细管上实现的对映体分离的分辨率高达 16.1。获得了模板的强识别能力（选择性因子为 3.23）和高柱性能（理论塔板数为 26,053 板/m）。还使用 S-萘普生或 S-酮洛芬作为模板分子制备了 MIP 涂层。对映体分离的分辨率分别为 2.20 和 4.56。结果表明，使用单官能单体合成 MIP 不仅是一个实验简化的过程，而且是一种生产高效和选择性手性固定相的方法。
