Barrett I D, Panesar N S, Burrow H M, Bird C C, Cawley J C, Child J A, Roberts B E
Clin Lab Haematol. 1982;4(3):285-97. doi: 10.1111/j.1365-2257.1982.tb00078.x.
The glucocorticoid binding properties and cytolethal responsiveness of leukaemic cells were studied in vitro in seven patients with hairy-cell leukaemia (HCL) and five with chronic lymphocytic leukaemia (CLL). Substantial levels of glucocorticoid binding were detected both in whole cell and cytosol preparations from all patients although the level of binding by HCL cells always exceeded that of CLL cells (P less than 0.05). In both leukaemic cell types the uptake and binding of prednisolone in vitro was significantly greater than that of dexamethasone (P less than 0.05). CLL cells showed a variable dose-related cytolethal response to methylprednisolone sodium succinate (MPSS) treatment in vitro although cytolytic effects were not marked in the usual pharmacological dose range (10(-5)-10(-6)M). Treatment of CLL patients with conventional doses of prednisone for extended periods or high intravenous infusions of MPSS over shorter periods had no consistent effect on the in-vitro level of steroid binding or the cytolethal responsiveness of CLL cells to glucocorticoid treatment. Although HCL cells proved highly resistant to the cytolethal effects of MPSS in vitro, the substantial binding of glucocorticoids by leukaemic cells from all HCL patients indicates the potential value of steroid therapy in this disease should be explored further.
对7例毛细胞白血病（HCL）患者和5例慢性淋巴细胞白血病（CLL）患者的白血病细胞进行了体外糖皮质激素结合特性和细胞致死反应性研究。在所有患者的全细胞和胞浆制剂中均检测到了大量的糖皮质激素结合，尽管HCL细胞的结合水平始终超过CLL细胞（P<0.05）。在两种白血病细胞类型中，泼尼松龙在体外的摄取和结合均显著大于地塞米松（P<0.05）。CLL细胞对琥珀酸钠甲泼尼龙（MPSS）体外治疗表现出可变的剂量相关细胞致死反应，尽管在通常的药理剂量范围（10^(-5)-10^(-6)M）内细胞溶解作用并不明显。用常规剂量的泼尼松长期治疗CLL患者或在较短时间内静脉高剂量输注MPSS对CLL细胞的体外类固醇结合水平或对糖皮质激素治疗的细胞致死反应性没有一致的影响。尽管HCL细胞在体外对MPSS的细胞致死作用具有高度抗性，但所有HCL患者白血病细胞对糖皮质激素的大量结合表明，应进一步探索类固醇疗法在该疾病中的潜在价值。
