Kuhn H Georg, Cooper-Kuhn Christiana M
Center for Brain Repair and Rehabilitation, Institute for Neuroscience and Physiology, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden.
Curr Pharm Biotechnol. 2007 Jun;8(3):127-31. doi: 10.2174/138920107780906531.
Bromodeoxyuridine (BrdU) is widely used for labeling dividing cells to determine their fate. In particular, the analysis of neurogenesis in the adult mammalian brain has made significant progress through the use of this technique. However; when using BrdU for labeling, there are several issues to consider in order to minimalize possible cytotoxicity or false-positive labeling. This current review summarizes methodological and technical aspects of BrdU administration and detection, compares alternative methods and gives recommendations on how to avoid labeling artifacts.
溴脱氧尿苷（BrdU）被广泛用于标记分裂细胞以确定其命运。特别是，通过使用该技术，成年哺乳动物大脑中的神经发生分析取得了重大进展。然而，在使用BrdU进行标记时，为了将可能的细胞毒性或假阳性标记降至最低，有几个问题需要考虑。本综述总结了BrdU给药和检测的方法学和技术方面，比较了替代方法，并就如何避免标记假象给出了建议。
