Taupin Philippe
National Neuroscience Institute, National University of Singapore and Nanyang Technological University, Singapore.
Regen Med. 2007 Jan;2(1):51-62. doi: 10.2217/17460751.2.1.51.
The first evidence that neurogenesis occurs in the adult brain was reported in rodents in the early 1960s, using [(3)H]-thymidine autoradiography. In the 1980s and 90s, the advent of new techniques and protocols for studying cell proliferation in situ, and particularly bromodeoxyuridine labeling, helped to confirm that neurogenesis occurs in the adult brain and neural stem cells reside in the adult CNS, including in humans. Bromodeoxyuridine labeling is currently the method most commonly used for studying neurogenesis in the adult brain. However, this procedure is not without limitations, and controversies. In this article, I will review recent protocols for studying adult neurogenesis, particularly new protocols for studying cell kinetics and cell proliferative history, using halopyrimidines. I will review these techniques, and discuss their implications for the field of adult neurogenesis.
20世纪60年代初，利用[（3）H] - 胸腺嘧啶核苷放射自显影技术，在啮齿动物中报道了成体大脑中发生神经发生的首个证据。在20世纪80年代和90年代，用于原位研究细胞增殖的新技术和方案的出现，尤其是溴脱氧尿苷标记法，有助于证实成体大脑中发生神经发生，并且神经干细胞存在于成体中枢神经系统中，包括人类。溴脱氧尿苷标记法目前是研究成体大脑神经发生最常用的方法。然而，该方法并非没有局限性和争议。在本文中，我将综述近期研究成体神经发生的方案，特别是使用卤代嘧啶研究细胞动力学和细胞增殖历史的新方案。我将对这些技术进行综述，并讨论它们对成体神经发生领域的意义。
