Kastrup Christian J, Shen Feng, Runyon Matthew K, Ismagilov Rustem F
Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA.
Biophys J. 2007 Oct 15;93(8):2969-77. doi: 10.1529/biophysj.107.109009. Epub 2007 Jun 22.
This article demonstrates that the threshold response of initiation of blood clotting to the size of a patch of stimulus is a robust phenomenon under a wide range of conditions and follows a simple scaling relationship based on the Damköhler number. Human blood and plasma were exposed to surfaces patterned with patches presenting clotting stimuli using microfluidics. Perturbations of the complex network of hemostasis, including temperature, variations in the concentration of stimulus (tissue factor), and the absence or inhibition of individual components of the network (factor IIa, factor V, factor VIII, and thrombomodulin), did not affect the existence of this response. A scaling relationship between the threshold patch size and the timescale of reaction for clotting was supported in numerical simulations, a simple chemical model system, and experiments with human blood plasma. These results may be useful for understanding the spatiotemporal dynamics of other autocatalytic systems and emphasize the relevance of clustering of proteins and lipids in the regulation of signaling processes.
本文表明，在广泛的条件下，血液凝固起始对刺激斑块大小的阈值响应是一种稳健的现象，并且遵循基于达姆科勒数的简单标度关系。使用微流体技术，将人血和血浆暴露于带有呈现凝血刺激斑块的图案化表面。止血复杂网络的扰动，包括温度、刺激物（组织因子）浓度的变化以及网络中单个成分（因子IIa、因子V、因子VIII和血栓调节蛋白）的缺失或抑制，均不影响这种响应的存在。在数值模拟、简单化学模型系统以及人血浆实验中，均支持了阈值斑块大小与凝血反应时间尺度之间的标度关系。这些结果可能有助于理解其他自催化系统的时空动态，并强调蛋白质和脂质聚集在信号传导过程调节中的相关性。
