Li Xiang, Chen Weiqiang, Li Zida, Li Ling, Gu Hongchen, Fu Jianping
Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI 48109, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Precision Instruments, Tsinghua University, Beijing 100084, China.
Trends Biotechnol. 2014 Nov;32(11):586-594. doi: 10.1016/j.tibtech.2014.09.003. Epub 2014 Oct 2.
The available techniques for assessing blood cell functions are limited considering the various types of blood cell and their diverse functions. In the past decade, rapid advances in microengineering have enabled an array of blood cell functional measurements that are difficult or impossible to achieve using conventional bulk platforms. Such miniaturized blood cell assay platforms also provide the attractive capabilities of reducing chemical consumption, cost, and assay time, as well as exciting opportunities for device integration, automation, and assay standardization. This review summarizes these contemporary microengineered tools and discusses their promising potential for constructing accurate in vitro models and rapid clinical diagnosis using minimal amounts of whole-blood samples.
考虑到血细胞的各种类型及其多样的功能，现有的评估血细胞功能的技术是有限的。在过去十年中，微工程的快速发展使得一系列血细胞功能测量成为可能，而这些测量使用传统的宏观平台很难或无法实现。这种小型化的血细胞检测平台还具有减少化学试剂消耗、成本和检测时间的吸引力，以及设备集成、自动化和检测标准化的令人兴奋的机会。本文综述了这些当代微工程工具，并讨论了它们在使用微量全血样本构建准确的体外模型和快速临床诊断方面的潜在前景。
