Amu S, Tarkowski A, Dörner T, Bokarewa M, Brisslert M
Department of Rheumatology and Inflammation Research, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden;Charité Universitätsmedizin Berlin and German Centre for Rheumatic Research, Berlin, Germany.
Scand J Immunol. 2007 Jul;66(1):77-86. doi: 10.1111/j.1365-3083.2007.01946.x.
We have shown that human CD20(+)25(+) B cells display immunomodulatory properties. The aim of this study was to investigate if CD25(+) B cells are found within the CD27 memory B cell population, and to analyse pattern of their cytokine production. B cells isolated from healthy subjects, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients were analysed regarding the frequency of CD25(+) B cells within certain B cell subsets. Purified CD25(+) B cells from healthy subject were used in vitro to evaluate their production of immunomodulatory cytokines. In healthy subjects the majority (60%) of memory B cells (CD20(+)27(+)) also co-expressed CD25 while only 10-20% of the naïve B cells (CD20(+)27(-)) and plasmablasts (CD20-27(+)) expressed CD25. In RA and SLE patients, we found that 51% and 48%, respectively, co-expressed CD25 in the memory population, whereas only 11% and 9% co-expressed CD25 in the naïve B cell population. Phenotypic analysis of the CD20(+)25(+)27(+) and CD20(+)25(+)27(-) cells using CD10, CD24, CD38, CD45, CD71, CD80, CD86, CD95, CD138, BAFF-R, TACI, IgA, IgD, IgG and IgM showed that CD20(+)25(+)27(+) B cells preferentially represent highly activated, Ig class switched memory B cells. Cytokine profile analysis showed that CD25(+) B cells secreted significantly higher levels of IL-10 versus CD25(-) B cells. In contrast, TGF-beta1 secretion was similar between the CD25(+) and CD25(-) sub-populations. In conclusion, CD20(+)25(+) B cells constitute a unique subpopulation preferentially occurring among CD20(+)27(+) memory B cells. We suggest that CD25 can be used as a marker for a memory B cell subset.
我们已经证明，人类CD20(+)25(+) B细胞具有免疫调节特性。本研究的目的是调查CD25(+) B细胞是否存在于CD27记忆B细胞群体中，并分析其细胞因子产生模式。对从健康受试者、类风湿性关节炎（RA）和系统性红斑狼疮（SLE）患者中分离出的B细胞，分析了特定B细胞亚群中CD25(+) B细胞的频率。使用从健康受试者中纯化的CD25(+) B细胞进行体外实验，以评估其免疫调节细胞因子的产生。在健康受试者中，大多数（60%）记忆B细胞（CD20(+)27(+)）也共表达CD25，而只有10 - 20%的幼稚B细胞（CD20(+)27(-)）和成浆细胞（CD20-27(+)）表达CD25。在RA和SLE患者中，我们发现记忆群体中分别有51%和48%共表达CD25，而在幼稚B细胞群体中分别只有11%和9%共表达CD25。使用CD10、CD24、CD38、CD45、CD71、CD80、CD86、CD95、CD138、BAFF-R、TACI、IgA、IgD、IgG和IgM对CD20(+)25(+)27(+)和CD20(+)25(+)27(-)细胞进行表型分析，结果显示CD20(+)25(+)27(+) B细胞优先代表高度活化、发生Ig类别转换的记忆B细胞。细胞因子谱分析表明，与CD25(-) B细胞相比，CD25(+) B细胞分泌的IL-10水平显著更高。相比之下，CD25(+)和CD25(-)亚群之间的TGF-β1分泌相似。总之，CD20(+)25(+) B细胞构成了一个独特的亚群，优先出现在CD20(+)27(+)记忆B细胞中。我们建议CD25可作为记忆B细胞亚群的标志物。
