聚乙二醇化干扰素-α加牛磺酸治疗大鼠肝纤维化
Pegylated interferon-alpha plus taurine in treatment of rat liver fibrosis.
作者信息
Tasci Ilker, Mas Mehmet Refik, Vural Sevil Atalay, Deveci Salih, Comert Bilgin, Alcigir Gunay, Mas Nuket, Akay Cemal, Bozdayi Mithat, Yurdaydin Cihan, Bozkaya Hakan, Uzunalimoglu Ozden, Isik Ahmet Turan, Said Harun M
机构信息
Department of Internal Medicine, Gulhane School of Medicine Etlik 06018 Ankara, Turkey.
出版信息
World J Gastroenterol. 2007 Jun 21;13(23):3237-44. doi: 10.3748/wjg.v13.i23.3237.
AIM
To investigate the antifibrotic effects of peginterferon-alpha 2b and taurine on oxidative stress markers and hepatocellular apoptosis.
METHODS
Sixty rats with CCl4-induced liver fibrosis were divided into 4 groups (n=15). Group 1 was left for spontaneous recovery (SR). Groups 2-4 received peginterferon-alpha 2b, taurine, and their combination, respectively, for four weeks. Histological fibrosis scores, histomorphometric analysis, tissue hydroxyproline, tissue MDA, GPx and SOD activities were determined. Activated stellate cells and hepatocellular apoptosis were also evaluated.
RESULTS
The degree of fibrosis decreased in all treatment groups compared to spontaneous recovery group. Taurine alone and in combination with peginterferon-alpha 2b reduced oxidative stress markers, but peginterferon-alpha 2b alone did not. Apoptotic hepatocytes and activated stellate cells were higher in groups 2-4 than in group 1. Combined taurine and peginterferon-alpha 2b further reduced fibrosis and increased activated stellate cell apoptosis, but could not improve oxidative stress more than taurine alone.
CONCLUSION
Peginterferon-alpha 2b exerts anti-fibrotic effects on rat liver fibrosis. It seems ineffective against oxidative stress in vivo. Peginterferon-alpha 2b in combination with taurine seems to be an antifibrotic strategy.
目的
研究聚乙二醇化干扰素α-2b和牛磺酸对氧化应激标志物及肝细胞凋亡的抗纤维化作用。
方法
将60只四氯化碳诱导的肝纤维化大鼠分为4组（每组n = 15）。第1组留作自然恢复（SR）。第2 - 4组分别接受聚乙二醇化干扰素α-2b、牛磺酸及其组合治疗，为期四周。测定组织学纤维化评分、组织形态计量分析、组织羟脯氨酸、组织丙二醛、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）活性。还评估了活化的星状细胞和肝细胞凋亡情况。
结果
与自然恢复组相比，所有治疗组的纤维化程度均降低。单独使用牛磺酸及其与聚乙二醇化干扰素α-2b联合使用可降低氧化应激标志物，但单独使用聚乙二醇化干扰素α-2b则无此作用。第2 - 4组的凋亡肝细胞和活化星状细胞高于第1组。牛磺酸与聚乙二醇化干扰素α-2b联合使用进一步减轻了纤维化并增加了活化星状细胞凋亡，但在改善氧化应激方面并不比单独使用牛磺酸更有效。
结论
聚乙二醇化干扰素α-2b对大鼠肝纤维化具有抗纤维化作用。它在体内似乎对氧化应激无效。聚乙二醇化干扰素α-2b与牛磺酸联合使用似乎是一种抗纤维化策略。
Zotero 插件