牛磺酸治疗四氯化碳诱导的肝损伤后肝细胞的超微结构变化。
Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury.
作者信息
Tasci Ilker, Mas Nuket, Mas Mehmet-Refik, Tuncer Murvet, Comert Bilgin
机构信息
Department of Internal Medicine, Gulhane School of Medicine, Etlik, Ankara, Turkey.
出版信息
World J Gastroenterol. 2008 Aug 21;14(31):4897-902. doi: 10.3748/wjg.14.4897.
Abstract
AIM
To search the organelle based changes in hepatocytes after taurine treatment in experimental liver fibrosis induced by CCl(4) administration.
METHODS
Thirty rats were divided into two groups. Group 1 (n = 15) was injected with CCl(4) plus taurine and Group 2 (n = 15) with CCl(4) plus saline for 12 wk. At the end of 12th wk, mitochondria, rough and smooth endoplasmic reticulum, and nuclei of hepatocytes were evaluated using a scoring system. The results were compared with histopathological findings, as well.
RESULTS
Taurine treatment reduced fibrosis scores significantly as compared to placebo. Organelle injury scores decreased significantly with taurine treatment. Ultrastructural and histopathological scores in both groups were in strong correlation (r = 0.931 for CCl(4) plus taurine and r = 0.899 for CCl(4) plus saline group).
CONCLUSION
Organelle based transmission electron microscopy findings can reflect successfully histological results as well as tissue healing in hepatocytes from hepatotoxin-induced liver fibrosis.
摘要
目的
探究在四氯化碳诱导的实验性肝纤维化中，牛磺酸处理后肝细胞内基于细胞器的变化。
方法
将30只大鼠分为两组。第1组（n = 15）注射四氯化碳加牛磺酸，第2组（n = 15）注射四氯化碳加生理盐水，持续12周。在第12周结束时，使用评分系统评估肝细胞的线粒体、粗面和滑面内质网以及细胞核。结果也与组织病理学结果进行了比较。
结果
与安慰剂相比，牛磺酸处理显著降低了纤维化评分。牛磺酸处理后细胞器损伤评分显著降低。两组的超微结构和组织病理学评分呈强相关（四氯化碳加牛磺酸组r = 0.931，四氯化碳加生理盐水组r = 0.899）。
结论
基于细胞器的透射电子显微镜检查结果能够成功反映肝毒素诱导的肝纤维化中肝细胞的组织学结果以及组织愈合情况。
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