Wang Zheng, Liu Wanshun, Han Baoqin, Yao Ruyong, Wei Changzheng
Institute of Oceanology,The Chinese Academy of Sciences, Qingdao 266071, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2007 Apr;24(2):340-4.
Effects of carboxymethyl-chitosan (CM-Chitosan) with different molecular weight on the proliferation of skin fibroblasts and keratinocytes were examined in vitro; bFGF and EGF, as controls, were seperately used for comparison. Chitosan with different molecular weight was prepared by acid degradation and oxidation degradation; CM-Chitosan with different molecular weight was synthesized from corresponding Chitosan. Microscopy and MTT method were applied to evaluate the different effects. The results demonstrated that CM-Chitosan with different molecular weight promoted the proliferation of skin fibroblasts and keratinocytes at 1-1000 ppm, and the concentration at 100 ppm had the strongest effects. The effects of low molecular weight CM-Chitosan were greater than those of high molecular weight CM-Chitosan. CM-Chitosan (Mn= 3KD) had the strongest promotive effects on skin fibroblasts and keratinocytes; it had equivalent effects when compared with bFGF and EGF.
体外研究了不同分子量的羧甲基壳聚糖（CM-壳聚糖）对皮肤成纤维细胞和角质形成细胞增殖的影响；分别使用碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）作为对照进行比较。通过酸降解和氧化降解制备了不同分子量的壳聚糖；从相应的壳聚糖合成了不同分子量的CM-壳聚糖。应用显微镜检查和MTT法评估不同的作用效果。结果表明，不同分子量的CM-壳聚糖在1-1000 ppm时促进皮肤成纤维细胞和角质形成细胞的增殖，100 ppm的浓度效果最强。低分子量CM-壳聚糖的作用效果大于高分子量CM-壳聚糖。CM-壳聚糖（Mn = 3KD）对皮肤成纤维细胞和角质形成细胞具有最强的促进作用；与bFGF和EGF相比，其效果相当。
