Pei P, Cigler C, Vonderschmitt D J
Institut für Klinische Chemie, Universitätsspital Zürich.
Schweiz Med Wochenschr. 1991 Dec 14;121(50):1883-6.
In the hospital clinical laboratory, plasma potassium and sodium are usually determined by using a flame photometer or ion-selective electrodes, for which special equipment is required. The new enzymatic methods of sodium and potassium can be used as a routine chemical method. We evaluated the new enzymatic methods for the determination of sodium and potassium in human plasma on the Hitachi 747. The sodium and potassium assay kits were purchased from Boehringer Mannheim, Germany. Precision studies were performed using three levels of pool plasma. The coefficients of variation (CV) of sodium and potassium determination were less than 1.0% and less than 2.2% by intraassay and interassay respectively. The recoveries of the assigned values of the ten control sera were 97-102%. Comparisons with results either from flame photometry and from ion-selective electrodes showed no clinically relevant differences for 100 patient samples. Performance of the enzymatic methods for Na+ and K+ is clinically comparable to flame photometry or ion-selective electrodes in the routine clinical chemistry laboratory.
在医院临床实验室中，血浆钾和钠通常采用火焰光度计或离子选择电极进行测定，而这需要特殊设备。钠和钾的新酶法可作为常规化学方法使用。我们在日立747上评估了测定人血浆中钠和钾的新酶法。钠和钾检测试剂盒购自德国宝灵曼公司。使用三种水平的混合血浆进行精密度研究。钠和钾测定的批内变异系数（CV）分别小于1.0%，批间变异系数小于2.2%。十个对照血清赋值的回收率为97 - 102%。与火焰光度法和离子选择电极法的结果比较显示，100份患者样本在临床上无显著差异。在常规临床化学实验室中，钠和钾酶法的性能在临床上与火焰光度法或离子选择电极法相当。
