Hübl W, Wejbora R, Shafti-Keramat I, Haider A, Hajdusich P, Bayer P M
Central Lab, Wilhelminenspital, Vienna, Austria.
Clin Chem. 1994 Aug;40(8):1528-31.
We evaluated the effect of hemolysis, icteric discoloration, lipemia, paraproteinemia, and uremia on enzymatic methods for determining sodium, potassium, and chloride, according to the National Committee for Clinical Laboratory Standards EP7-P proposals for testing interference from endogenous substances. The sodium, potassium, and chloride assays (reagent kits supplied by Boehringer Mannheim) were based on electrolyte-dependent beta-galactosidase, pyruvate kinase, and alpha-amylase, respectively. The results were compared with those obtained by indirect ion-selective electrodes (ISE), which in turn had been validated by flame photometry. We analyzed the samples with Hitachi 717, 737, and 911 chemistry analyzers and with an IL943 flame photometer. The enzymatic results were in good agreement with those by ISE, the interference-related differences generally being without clinical significance; however, none of the enzymatic methods could analyze grossly lipemic samples.
根据美国国家临床实验室标准委员会（NCCLS）EP7-P中关于检测内源性物质干扰的提议，我们评估了溶血、黄疸、脂血、副蛋白血症和尿毒症对钠、钾、氯酶法测定的影响。钠、钾、氯检测（由罗氏公司提供的试剂盒）分别基于依赖电解质的β-半乳糖苷酶、丙酮酸激酶和α-淀粉酶。将结果与间接离子选择电极（ISE）法获得的结果进行比较，而ISE法又已经通过火焰光度法验证。我们使用日立717、737和911化学分析仪以及IL943火焰光度计对样本进行分析。酶法结果与ISE法结果高度一致，与干扰相关的差异通常无临床意义；然而，所有酶法均无法分析严重脂血样本。
