Ehrhardt V, Baadenhuijsen H, Brenna S, Browne M, Garcia-Beltrán L, Hellsing K, Külpmann W R, Paschen K, Pascual-Mostaza C, Pei P
Boehringer Mannheim GmbH, Bundesrepublik Deutschland.
Wien Klin Wochenschr Suppl. 1992;192:12-21.
We present the results of a multicentre evaluation with Boehringer Mannheim/Hitachi instruments of new "enzymatic" methods for the determination of Na+, K+, and Cl- in serum or plasma. The between-day coefficient of variation was less than 1.4% (Na+), less than 2.6% (K+) and less than 1.7% (Cl-). The linear range of the assays were at least 80 to 200 mmol/l (Na+), 1.5 to 17 mmol/l (K+) and about 30 to at least 200 mmol/l (Cl-). The comparisons with routine flame atomic emission spectrometry and coulometry showed a satisfactory agreement of the test results. The "enzymatic" assays are insensitive to even grossly elevated levels of bilirubin and lipids (sodium, potassium, and chloride assays), NH4+ (potassium assay) and amylase (chloride assay). Interference by various drugs was not detected. Since the new methods can easily be adapted to photometric clinical chemistry instruments, they represent a valuable alternative to the use of ion-selective electrodes, flame atomic emission spectrometry and coulometry.
我们展示了使用勃林格殷格翰/日立仪器对血清或血浆中钠、钾和氯的新型“酶法”测定方法进行多中心评估的结果。日间变异系数小于1.4%（钠）、小于2.6%（钾）和小于1.7%（氯）。测定的线性范围至少为80至200 mmol/L（钠）、1.5至17 mmol/L（钾）和约30至至少200 mmol/L（氯）。与常规火焰原子发射光谱法和库仑法的比较显示测试结果具有令人满意的一致性。“酶法”测定对胆红素和脂质（钠、钾和氯测定）、铵离子（钾测定）和淀粉酶（氯测定）的严重升高水平不敏感。未检测到各种药物的干扰。由于新方法可以轻松适用于光度临床化学仪器，它们是使用离子选择性电极、火焰原子发射光谱法和库仑法的有价值替代方法。
