Kleine T O
Dtsch Med Wochenschr. 1981 Jul 3;106(27):865-70. doi: 10.1055/s-2008-1070416.
The vital staining method using prestained slides permits satisfactory characterization and differentiation of benign and malignant cells present in CSF using 10 and 20 mul of CSF. In comparison the sedimentation technique of Sayk and the cytocentrifuge with subsequent Pappenheim staining require larger volumes of CSF. They have the disadvantage of possible loss of cells and of cell denaturation.
使用预染色载玻片的活体染色方法能够使用10微升和20微升脑脊液对脑脊液中存在的良性和恶性细胞进行令人满意的表征和鉴别。相比之下,赛克的沉降技术以及随后进行帕彭海姆染色的细胞离心涂片法需要更大体积的脑脊液。它们存在细胞可能丢失和细胞变性的缺点。