Tan Hua, Huang Shisheng, Yang Kun-Lin
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576.
Langmuir. 2007 Jul 31;23(16):8607-13. doi: 10.1021/la701258c. Epub 2007 Jun 26.
In this paper, we report a method of transferring complementary target DNA from an aqueous solution onto a solid surface by using affinity microcontact printing. In this approach, the probe DNA is first immobilized on the surface of an aminated poly(dimethylsiloxane) (PDMS) stamp. After a complementary target DNA hybridizes with the probe DNA on the stamp surface, the PDMS stamp is printed on an aminated glass slide. By using fluorescent microscopy, we show that only complementary target DNA, but not noncomplementary DNA, can be captured onto the surface of the stamp and then transferred to the aminated glass slide. The transfer of DNA can be attributed to the stronger electrostatic attraction between DNA and amine groups compared to the hydrogen bonds between the hybridized DNA molecules. We also investigate several factors that may influence the transfer of DNA, such as the surface density of amine groups, hybridization conditions, and contamination from unreacted PDMS monomers.
在本文中，我们报道了一种通过亲和微接触印刷将互补靶DNA从水溶液转移到固体表面的方法。在这种方法中，首先将探针DNA固定在胺化聚二甲基硅氧烷（PDMS）印章的表面。当互补靶DNA与印章表面的探针DNA杂交后，将PDMS印章印在胺化玻璃载玻片上。通过荧光显微镜观察，我们发现只有互补靶DNA，而非非互补DNA，能够被捕获到印章表面，然后转移到胺化玻璃载玻片上。DNA的转移可归因于DNA与胺基之间比杂交DNA分子之间的氢键更强的静电吸引力。我们还研究了几个可能影响DNA转移的因素，如胺基的表面密度、杂交条件以及未反应的PDMS单体的污染。
