一种用于固定寡核苷酸刷的微接触印刷方法。
A microcontact printing approach to the immobilization of oligonucleotide brushes.
作者信息
Razumovitch Julia, Meier Wolfgang, Vebert Corinne
机构信息
Department of Chemistry, University of Basel, Klingelbergstrasse 80, Basel 4056, Switzerland.
出版信息
Biophys Chem. 2009 Jan;139(1):70-4. doi: 10.1016/j.bpc.2008.10.005. Epub 2008 Oct 29.
PMID:19004539
Abstract
Solution hybridized oligonucleotides were immobilized onto surfaces via micro-contact printing. Besides micro-patterning of the substrate, sequential dehybridization and rehybridization were monitored via laser scanning microscopy, which assess the surface tethering of the oligonucleotides into a brush.
摘要
通过微接触印刷将溶液杂交的寡核苷酸固定在表面上。除了对底物进行微图案化之外，还通过激光扫描显微镜监测连续的解杂交和再杂交过程，该显微镜评估寡核苷酸在表面上形成刷状的连接情况。
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