Razumovitch Julia, Meier Wolfgang, Vebert Corinne
Department of Chemistry, University of Basel, Klingelbergstrasse 80, Basel 4056, Switzerland.
Biophys Chem. 2009 Jan;139(1):70-4. doi: 10.1016/j.bpc.2008.10.005. Epub 2008 Oct 29.
Solution hybridized oligonucleotides were immobilized onto surfaces via micro-contact printing. Besides micro-patterning of the substrate, sequential dehybridization and rehybridization were monitored via laser scanning microscopy, which assess the surface tethering of the oligonucleotides into a brush.
通过微接触印刷将溶液杂交的寡核苷酸固定在表面上。除了对底物进行微图案化之外，还通过激光扫描显微镜监测连续的解杂交和再杂交过程，该显微镜评估寡核苷酸在表面上形成刷状的连接情况。
