Miller K, Reich E, Rück A, Hautmann R
University of Ulm, Department of Urology, FRG.
Urol Res. 1991;19(6):353-6. doi: 10.1007/BF00310149.
The uptake of photosan and the intracellular sites of photoradiation-induced damage were investigated in vitro in bladder carcinoma cells and in normal bladder cells. Cells were examined by phase contrast, fluorescence and electron microscopy. The concentration of photosan, measured in microgram/10(6) cells, showed a good correlation to the incubation time. At all incubation times, control cells showed a lower uptake when compared with tumor cells. Following photodynamic therapy (PDT), phase-contrast microscopy revealed marked changes in tumor cells, whereas only minor effects could be detected at the cell membrane of the control cells. Following PDT, most of the investigated cells showed changes of the mitochondria and cytoplasma. These changes consisted of dissolution of the cristae, predominantly in the central part of the mitochondria. Twenty-four hours after PDT the shape of the mitochondria had changed markedly and the cristae were found to be completely destroyed. Moreover, the cystoplasma showed numerous vacuoles, and the number of mitochondria was decreased compared to non-treated cells.
在体外对膀胱癌细胞和正常膀胱细胞进行了光神（Photosan）摄取及光辐射诱导损伤的细胞内位点研究。通过相差显微镜、荧光显微镜和电子显微镜对细胞进行检查。以微克/10⁶细胞为单位测量的光神浓度与孵育时间呈现良好的相关性。在所有孵育时间，与肿瘤细胞相比，对照细胞的摄取量较低。光动力疗法（PDT）后，相差显微镜显示肿瘤细胞有明显变化，而对照细胞的细胞膜仅检测到轻微影响。PDT后，大多数被研究细胞的线粒体和细胞质出现变化。这些变化包括嵴溶解，主要发生在线粒体中央部分。PDT后24小时，线粒体形状发生明显改变，嵴被发现完全破坏。此外，细胞质出现大量空泡，与未处理细胞相比，线粒体数量减少。
