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用于巴尔通体属血清分型的微阵列

Microarray for serotyping of Bartonella species.

作者信息

Bonhomme Cyrille J, Nappez Claude, Raoult Didier

机构信息

Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine de Marseille, Marseille cedex 05, France.

出版信息

BMC Microbiol. 2007 Jun 25;7:59. doi: 10.1186/1471-2180-7-59.

DOI:10.1186/1471-2180-7-59
PMID:17593301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3225882/
Abstract

BACKGROUND

Bacteria of the genus Bartonella are responsible for a large variety of human and animal diseases. Serological typing of Bartonella is a method that can be used for differentiation and identification of Bartonella subspecies.

RESULTS

We have developed a novel multiple antigenic microarray to serotype Bartonella strains and to select poly and monoclonal antibodies. It was validated using mouse polyclonal antibodies against 29 Bartonella strains. We then tested the microarray for serotyping of Bartonella strains and defining the profile of monoclonal antibodies. Bartonella strains gave a strong positive signal and all were correctly identified. Screening of monoclonal antibodies towards the Gro EL protein of B. clarridgeiae identified 3 groups of antibodies, which were observed with variable affinities against Bartonella strains.

CONCLUSION

We demonstrated that microarray of spotted bacteria can be a practical tool for serotyping of unidentified strains or species (and also for affinity determination) by polyclonal and monoclonal antibodies. This could be used in research and for identification of bacterial strains.

摘要

背景

巴尔通体属细菌可引发多种人类和动物疾病。巴尔通体的血清学分型是一种可用于区分和鉴定巴尔通体亚种的方法。

结果

我们开发了一种新型多重抗原微阵列，用于巴尔通体菌株的血清分型和多克隆及单克隆抗体的筛选。使用针对29株巴尔通体菌株的小鼠多克隆抗体对其进行了验证。然后我们测试了该微阵列用于巴尔通体菌株血清分型和确定单克隆抗体谱的能力。巴尔通体菌株给出了强阳性信号，所有菌株均被正确鉴定。针对克拉氏巴尔通体Gro EL蛋白的单克隆抗体筛选鉴定出3组抗体，这些抗体对巴尔通体菌株的亲和力各不相同。

结论

我们证明，斑点细菌微阵列可以成为一种实用工具，通过多克隆和单克隆抗体对未鉴定的菌株或物种进行血清分型（以及用于亲和力测定）。这可用于研究和细菌菌株的鉴定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/0dc6323c22ab/1471-2180-7-59-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/f3e4f0ccca46/1471-2180-7-59-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/d210656cf31e/1471-2180-7-59-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/d9c99dd03172/1471-2180-7-59-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/11d85ae99b40/1471-2180-7-59-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/8193b5276ec7/1471-2180-7-59-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/0dc6323c22ab/1471-2180-7-59-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/f3e4f0ccca46/1471-2180-7-59-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/d210656cf31e/1471-2180-7-59-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/d9c99dd03172/1471-2180-7-59-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/11d85ae99b40/1471-2180-7-59-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/8193b5276ec7/1471-2180-7-59-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/3225882/0dc6323c22ab/1471-2180-7-59-2.jpg

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