Servicio de Microbiología, Hospital Donostia, San Sebastián, Spain.
J Microbiol Methods. 2010 Mar;80(3):274-80. doi: 10.1016/j.mimet.2010.01.011. Epub 2010 Jan 20.
The use of antibody-based miniaturized devices for microbiological applications is a field poorly investigated in the era of more developed molecular amplification techniques. A novel antibody microarray for Streptococcus pneumoniae serotyping was developed, by printing nanolitre volumes of pneumococcal serotype-specific antibodies on multi-well slides. This microarray, which showed high specificity when tested against reference and clinical S. pneumoniae isolates, can be applicable as a faster, cost-effective and accurate serotyping technique for pneumococcal epidemiological studies.
基于抗体的微型设备在微生物学应用中的使用在更发达的分子扩增技术时代是一个研究甚少的领域。通过在多孔板上打印纳升级别的肺炎链球菌血清型特异性抗体，开发了一种新型的肺炎链球菌血清分型抗体微阵列。该微阵列在针对参考和临床分离的肺炎链球菌进行测试时显示出很高的特异性，可作为一种更快、更具成本效益和更准确的肺炎链球菌血清分型技术应用于肺炎链球菌的流行病学研究。
