Polzin Gregory M, Kosa-Maines Rachel E, Ashley David L, Watson Clifford H
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, USA.
Environ Sci Technol. 2007 Feb 15;41(4):1297-302. doi: 10.1021/es060609l.
Mainstream cigarette smoke is a complex aerosol containing more than 4400 chemicals. The proliferation of new brands has necessitated development of faster and more reliable methods capable of analyzing a wide range of compounds in cigarette smoke. Although the International Agency for Research on Cancer has classified whole cigarette smoke as a human carcinogen, many of the individual chemicals are themselves highly biologically active as carcinogens, teratogens, or have implications for cardiovascular disease. Among these chemicals are many volatile organic compounds (VOCs), e.g., benzene, ethylbenzene, and styrene. To analyze VOCs in mainstream cigarette smoke, we developed a novel headspace collection technique using polyvinylfluoride bags for sample collection followed by cannula transfer to evacuated standard 20-mL auto sampler vials. Coupling collection of the vapor-phase cigarette smoke with automated analysis by solid-phase microextraction and gas chromatography/mass spectrometry enabled us to routinely quantify selected VOCs in mainstream cigarette smoke. This technique has similar reproducibility to previous cold trap and impinger collection methods with significantly higher sample throughput and virtually no solvent waste. In this report we demonstrate the method's analytical capabilities by quantitatively analyzing 13 selected VOCs in mainstream cigarette smoke from top-selling domestic brands.
主流香烟烟雾是一种复杂的气溶胶，含有4400多种化学物质。新品牌香烟的大量涌现使得有必要开发更快、更可靠的方法，以便能够分析香烟烟雾中的多种化合物。尽管国际癌症研究机构已将整个香烟烟雾归类为人类致癌物，但许多单一化学物质本身作为致癌物、致畸剂具有很高的生物活性，或对心血管疾病有影响。这些化学物质中有许多挥发性有机化合物（VOCs），例如苯、乙苯和苯乙烯。为了分析主流香烟烟雾中的挥发性有机化合物，我们开发了一种新颖的顶空收集技术，使用聚氟乙烯袋收集样品，然后通过插管转移到抽空的20 mL标准自动进样器小瓶中。将气相香烟烟雾的收集与通过固相微萃取和气相色谱/质谱联用的自动分析相结合，使我们能够常规定量主流香烟烟雾中选定的挥发性有机化合物。该技术与以前的冷阱和冲击器收集方法具有相似的重现性，样品通量显著更高，几乎没有溶剂浪费。在本报告中，我们通过定量分析国内畅销品牌主流香烟烟雾中的13种选定挥发性有机化合物，展示了该方法的分析能力。
