Melnick J L, Courtney R J, Powell K L, Schaffer P A, Melnick M B, Dreesman G R, Anzai T, Adam E
Cancer Res. 1976 Feb;36(2 pt 2):845-56.
Virus-induced polypeptides of cells infected by herpes simplex virus (HSV) types 1 and 2 were investigated by analysis on polyacrylamide gels and by determination of their antigenicity. Some polypeptides, VP154 and VP134, had immunological reactivity common to both virus types, while others (VP175 and VP123) were type specific. Only the glycosylated polypeptides were able to induce neutralizing antibody. The expression of viral genetic information was studied in newborn mice infected with wild-type and ts mutant viruses; some mutants had become attenuated and had lost pathogenicity for newborn mice while others had not. From induction experiments in HSV=transformed hamster cells, it appears that detection of enhanced replication of ts mutants in human cancer cells would be an indication of resident HSV genetic information. Sera obtained from cancer patients were examined for antibodies to early proteins synthesized in HSV-infected cells. The method used was an indirect radioimmune precipitation test followed by polyacrylamide gel electrophoretic analysis of immune precipitates. Cervical cancer patients had sera with a higher reactivity to early nonstructural polypeptides than to breast cancer patients or to matched healthy women. In contrast to the results with early polypeptides, little difference was detectable between the matched sera in their reactivity with a major capsid polypeptide, which is synthesized late in the infectious cycle.
通过聚丙烯酰胺凝胶分析和抗原性测定，对感染1型和2型单纯疱疹病毒（HSV）的细胞中病毒诱导的多肽进行了研究。一些多肽，即VP154和VP134，具有两种病毒类型共有的免疫反应性，而其他多肽（VP175和VP123）具有型特异性。只有糖基化多肽能够诱导中和抗体。在感染野生型和温度敏感（ts）突变病毒的新生小鼠中研究了病毒遗传信息的表达；一些突变体已减毒并失去对新生小鼠的致病性，而其他突变体则没有。从HSV转化的仓鼠细胞中的诱导实验来看，在人类癌细胞中检测到ts突变体的复制增强将表明存在HSV遗传信息。检测了癌症患者血清中针对HSV感染细胞中合成的早期蛋白的抗体。所采用的方法是间接放射免疫沉淀试验，随后对免疫沉淀物进行聚丙烯酰胺凝胶电泳分析。宫颈癌患者血清对早期非结构多肽的反应性高于乳腺癌患者或匹配的健康女性。与早期多肽的结果相反，在与主要衣壳多肽的反应性方面，匹配血清之间几乎没有可检测到的差异，主要衣壳多肽是在感染周期后期合成的。
