Heilman C J, Zweig M, Stephenson J R, Hampar B
J Virol. 1979 Jan;29(1):34-42. doi: 10.1128/JVI.29.1.34-42.1979.
A polypeptide (p40) of approximately 40,000 molecular weight was isolated from herpes simplex virus type 1 and 2 nucleocapsids by gel filtration and ion exchange chromatography. This protein appears to be the same as protein 22a described previously (Gibson and Roizman, J. Virol. 10:1044--1052, 1972). Competition immunoassays were developed by using purified p40 and antisera prepared in guinea pigs. The assays indicated that the p40's from herpes simplex virus types 1 and 2 possess both type-specific and cross-reactive antigenic determinants. Antibodies to the p40 cross-reactive determinant reacted with antigens in simian herpes virus SA8-infected cells, but not with antigens induced by pseudorabies virus. Preliminary results indicated that a radioimmunoprecipitation test can be used to detect type-specific herpes simplex virus p40 antibodies in human sera.
通过凝胶过滤和离子交换色谱法从1型和2型单纯疱疹病毒核衣壳中分离出一种分子量约为40,000的多肽（p40）。这种蛋白质似乎与先前描述的蛋白质22a相同（吉布森和罗伊兹曼，《病毒学杂志》10:1044 - 1052，1972年）。利用纯化的p40和在豚鼠中制备的抗血清开发了竞争免疫测定法。这些测定表明，1型和2型单纯疱疹病毒的p40具有型特异性和交叉反应性抗原决定簇。针对p40交叉反应性决定簇的抗体与感染猿猴疱疹病毒SA8的细胞中的抗原发生反应，但不与伪狂犬病病毒诱导的抗原发生反应。初步结果表明，放射免疫沉淀试验可用于检测人血清中型特异性单纯疱疹病毒p40抗体。
